Literature DB >> 8575022

Integrative and replicative transformation of Penicillium canescens with a heterologous nitrate-reductase gene.

A Y Aleksenko1, N A Makarova, I V Nikolaev, A J Clutterbuck.   

Abstract

A wild isolate of Penicillium canescens was subjected to mutagenesis, and 150 chlorate-resistant mutants were isolated and classified in respect of their ability to utilize various nitrogen sources. Strains supposedly deficient in nitrate reductase have been transformed with the nitrate-reductase gene from Aspergillus niger. Transformation probably occurred by non-homologous integration of the transforming vector into the chromosome. Co-transformation with the AMA 1 replicating element from A. nidulans enhanced transformation frequency up to 2000-fold, and was shown to result in autonomous maintenance of replicating concatenates, one of which was re-isolated by transformation of E. coli.

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Year:  1995        PMID: 8575022     DOI: 10.1007/bf00310818

Source DB:  PubMed          Journal:  Curr Genet        ISSN: 0172-8083            Impact factor:   3.886


  11 in total

1.  A general method for isolation of high molecular weight DNA from eukaryotes.

Authors:  N Blin; D W Stafford
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

2.  Chlorate toxicity in Aspergillus nidulans. Studies of mutants altered in nitrate assimilation.

Authors:  D J Cove
Journal:  Mol Gen Genet       Date:  1976-07-23

3.  Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans.

Authors:  M J Daboussi; A Djeballi; C Gerlinger; P L Blaiseau; I Bouvier; M Cassan; M H Lebrun; D Parisot; Y Brygoo
Journal:  Curr Genet       Date:  1989-06       Impact factor: 3.886

Review 4.  Genetic studies of nitrate assimilation in Aspergillus nidulans.

Authors:  D J Cove
Journal:  Biol Rev Camb Philos Soc       Date:  1979-08

5.  Cointegration of transforming DNAs in Aspergillus nidulans: a model using autonomously-replicating plasmids.

Authors:  A Y Aleksenko
Journal:  Curr Genet       Date:  1994-10       Impact factor: 3.886

6.  An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency.

Authors:  D Gems; I L Johnstone; A J Clutterbuck
Journal:  Gene       Date:  1991-02-01       Impact factor: 3.688

7.  Co-transformation with autonomously-replicating helper plasmids facilitates gene cloning from an Aspergillus nidulans gene library.

Authors:  D H Gems; A J Clutterbuck
Journal:  Curr Genet       Date:  1993-12       Impact factor: 3.886

8.  An 'instant gene bank' method for gene cloning by mutant complementation.

Authors:  D Gems; A Aleksenko; L Belenky; S Robertson; M Ramsden; Y Vinetski; A J Clutterbuck
Journal:  Mol Gen Genet       Date:  1994-02

9.  Transformation of Aspergillus niger with the homologous nitrate reductase gene.

Authors:  S E Unkles; E I Campbell; D Carrez; C Grieve; R Contreras; W Fiers; C A Van den Hondel; J R Kinghorn
Journal:  Gene       Date:  1989-05-15       Impact factor: 3.688

10.  Cloning an Aspergillus nidulans developmental gene by transformation.

Authors:  I L Johnstone; S G Hughes; A J Clutterbuck
Journal:  EMBO J       Date:  1985-05       Impact factor: 11.598

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  5 in total

1.  Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans.

Authors:  Saba Hasan; Rana Inder Singh; Sandhu Sardul Singh
Journal:  Indian J Microbiol       Date:  2011-01-25       Impact factor: 2.461

2.  Enhancement of the enzymatic cellulose saccharification by Penicillium verruculosum multienzyme cocktails containing homologously overexpressed lytic polysaccharide monooxygenase.

Authors:  Margarita V Semenova; Alexander V Gusakov; Pavel V Volkov; Veronika Yu Matys; Vitaly A Nemashkalov; Vadim D Telitsin; Aleksandra M Rozhkova; Arkady P Sinitsyn
Journal:  Mol Biol Rep       Date:  2019-02-15       Impact factor: 2.316

3.  Fungal artificial chromosomes for mining of the fungal secondary metabolome.

Authors:  Jin Woo Bok; Rosa Ye; Kenneth D Clevenger; David Mead; Megan Wagner; Amanda Krerowicz; Jessica C Albright; Anthony W Goering; Paul M Thomas; Neil L Kelleher; Nancy P Keller; Chengcang C Wu
Journal:  BMC Genomics       Date:  2015-04-29       Impact factor: 3.969

4.  Using an Inducible Promoter of a Gene Encoding Penicillium verruculosum Glucoamylase for Production of Enzyme Preparations with Enhanced Cellulase Performance.

Authors:  Alexander G Bulakhov; Pavel V Volkov; Aleksandra M Rozhkova; Alexander V Gusakov; Vitaly A Nemashkalov; Aidar D Satrutdinov; Arkady P Sinitsyn
Journal:  PLoS One       Date:  2017-01-20       Impact factor: 3.240

5.  Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens.

Authors:  Larisa Shcherbakova; Alexandra Rozhkova; Dmitrii Osipov; Ivan Zorov; Oleg Mikityuk; Natalia Statsyuk; Olga Sinitsyna; Vitaly Dzhavakhiya; Arkady Sinitsyn
Journal:  Toxins (Basel)       Date:  2020-07-25       Impact factor: 4.546

  5 in total

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