Literature DB >> 1313196

A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction.

P Kiene1, K Milde-Langosch, M Runkel, K Schulz, T Löning.   

Abstract

The use of chelating resin in a simple, rapid and efficient pre-treatment protocol to process formalin-fixed, paraffin-embedded specimens for the polymerase chain reaction (PCR) is described and compared to other pre-treatment techniques. With this modified PCR protocol, a variety of human autopsy and biopsy specimens were investigated for presence of DNA of human papilomaviruses, cytomegalovirus or Epstein-Barr virus. These viruses were detected in a productive or non-/low-productive state. Amplimers generated ranged from 88 to 450 base pairs length. Under the specified technical conditions, a considerable range of DNA molecules can be amplified from paraffin-embedded material, some older than 10 years.

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Year:  1992        PMID： 1313196     DOI： 10.1007/bf01600280

Source DB:  PubMed          Journal:  Virchows Arch A Pathol Anat Histopathol        ISSN： 0174-7398


  17 in total

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Journal:  J Clin Pathol       Date:  1990-06       Impact factor: 3.411

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Journal:  Intervirology       Date:  1988       Impact factor: 1.763

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Journal:  Am J Pathol       Date:  1990-03       Impact factor: 4.307

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Authors:  D K Shibata; N Arnheim; W J Martin
Journal:  J Exp Med       Date:  1988-01-01       Impact factor: 14.307
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  11 in total

1.  p53 is a persistent and predictive marker in advanced ovarian carcinomas: multivariate analysis including comparison with Ki67 immunoreactivity.

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3.  Retrospective determination of HIV-1 status by a PCR method on paraffin wax embedded sections.

Authors:  T Slavik; M Wolfaardt; H van Zyl; I W Simson
Journal:  J Clin Pathol       Date:  1995-08       Impact factor: 3.411

4.  Use of neuropathological tissue for molecular genetic studies: parameters affecting DNA extraction and polymerase chain reaction.

Authors:  S Kösel; M B Graeber
Journal:  Acta Neuropathol       Date:  1994       Impact factor: 17.088

5.  [Detection of human papillomavirus (HVP)-DNA in oral manifestation of lichen planus].

Authors:  M Vesper; S Riethdorf; E Christoph; A Ruthke; R Schmelzle; T Löning
Journal:  Mund Kiefer Gesichtschir       Date:  1997-05

6.  Comparison of benign and malignant endometrial lesions for their p53 state, using immunohistochemistry and temperature-gradient gel electrophoresis.

Authors:  L Riethdorf; C Begemann; S Riethdorf; K Milde-Langosch; T Löning
Journal:  Virchows Arch       Date:  1996-04       Impact factor: 4.064

7.  Pretreatment with restriction enzyme or bovine serum albumin for effective PCR amplification of Epstein-Barr virus DNA in DNA extracted from paraffin-embedded gastric carcinoma tissue.

Authors:  Y Satoh; N Takasaka; Y Hoshikawa; M Osaki; S Ohfuji; H Ito; N Kaibara; T Kurata; T Sairenji
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948

8.  Malignant lymphomas induced by an Epstein-Barr virus-related herpesvirus from Macaca arctoides--a rabbit model.

Authors:  P Wutzler; A Meerbach; I Färber; H Wolf; K Scheibner
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9.  Rapid techniques for DNA extraction from routinely processed archival tissue for use in PCR.

Authors:  R Sepp; I Szabó; H Uda; H Sakamoto
Journal:  J Clin Pathol       Date:  1994-04       Impact factor: 3.411

10.  Polymerase chain reaction-assisted papillomavirus detection in cervicovaginal smears: stratification by clinical risk and cytology reports.

Authors:  C Kühler-Obbarius; K Milde-Langosch; G Helling-Giese; A Salfelder; C Peimann; T Löning
Journal:  Virchows Arch       Date:  1994       Impact factor: 4.064
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