Literature DB >> 3009004

Southern blot analysis of DNA extracted from formalin-fixed pathology specimens.

L Dubeau, L A Chandler, J R Gralow, P W Nichols, P A Jones.   

Abstract

We have developed a method for the extraction of DNA from formalin-fixed, paraffin-embedded pathology specimens. High-molecular-weight DNA was recovered from well-fixed nonautolyzed samples of viable tissue. DNA recovered from samples exposed to picric acid or mercuric chloride containing fixatives was not intact. Increasing the formalin fixation time decreased the amount of intact DNA available. When these limitations were taken into consideration, the procedure allowed for the removal of degraded and chemically modified DNA from the preparation, and the final product was suitable for quantitative and qualitative analysis by Southern or dot blotting techniques. Digestion with methylation-sensitive restriction endonucleases showed that DNA methylation patterns were not altered after formalin fixation.

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Year:  1986        PMID： 3009004

Source DB:  PubMed          Journal:  Cancer Res        ISSN： 0008-5472            Impact factor:   12.701


  56 in total

1.  A simple method for PCR based analyses of immunohistochemically stained, microdissected, formalin fixed, paraffin wax embedded material.

Authors:  H E Alcock; T J Stephenson; J A Royds; D W Hammond
Journal:  Mol Pathol       Date:  1999-06

2.  Use of paraffin wax embedded bone marrow trephine biopsy specimens as a source of archival DNA.

Authors:  A B Provan; E Hodges; A G Smith; J L Smith
Journal:  J Clin Pathol       Date:  1992-09       Impact factor: 3.411

3.  A simple and rapid technique to process formalin-fixed, paraffin-embedded tissues for the detection of viruses by the polymerase chain reaction.

Authors:  P Kiene; K Milde-Langosch; M Runkel; K Schulz; T Löning
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1992

4.  DNA extraction from archival formalin-fixed, paraffin-embedded tissues: heat-induced retrieval in alkaline solution.

Authors:  Shan-Rong Shi; Ram Datar; Cheng Liu; Lin Wu; Zina Zhang; Richard J Cote; Clive R Taylor
Journal:  Histochem Cell Biol       Date:  2004-08-20       Impact factor: 4.304

5.  Molecular genetic analysis of DNA obtained from fixed, air dried or paraffin embedded sources.

Authors:  K Grünewald; J Lyons; T E Hansen-Hagge; J W Janssen; H Feichtinger; C R Bartram
Journal:  Ann Hematol       Date:  1991-04       Impact factor: 3.673

6.  Fundamental study on the mechanism of DNA degradation in tissues fixed in formaldehyde.

Authors:  Y Tokuda; T Nakamura; K Satonaka; S Maeda; K Doi; S Baba; T Sugiyama
Journal:  J Clin Pathol       Date:  1990-09       Impact factor: 3.411

7.  Conversions of formaldehyde-modified 2'-deoxyadenosine 5'-monophosphate in conditions modeling formalin-fixed tissue dehydration.

Authors:  Vladimir K Rait; Qingrong Zhang; Daniele Fabris; Jeffrey T Mason; Timothy J O'Leary
Journal:  J Histochem Cytochem       Date:  2005-08-22       Impact factor: 2.479

8.  Amplification of whole tumor genomes and gene-by-gene mapping of genomic aberrations from limited sources of fresh-frozen and paraffin-embedded DNA.

Authors:  Markus Bredel; Claudia Bredel; Dejan Juric; Young Kim; Hannes Vogel; Griffith R Harsh; Lawrence D Recht; Jonathan R Pollack; Branimir I Sikic
Journal:  J Mol Diagn       Date:  2005-05       Impact factor: 5.568

9.  Formalin-fixed paraffin-embedded tissue as a source for quantitation of carcinogen DNA adducts: aristolochic acid as a prototype carcinogen.

Authors:  Byeong Hwa Yun; Lihua Yao; Bojan Jelaković; Jovan Nikolić; Kathleen G Dickman; Arthur P Grollman; Thomas A Rosenquist; Robert J Turesky
Journal:  Carcinogenesis       Date:  2014-04-28       Impact factor: 4.944

10.  Monoclonal antibodies to human germ cell tumors from "routine" paraffin-embedded pathological specimens.

Authors:  A Takenaka; S Kitazawa; S Maeda; S Kamidono
Journal:  Histochemistry       Date:  1990
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