AIMS: This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC). METHODS AND RESULTS: Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk. CONCLUSIONS: PCR reactions containing one cell yielded a DPCR product. SIGNIFICANCE AND IMPACT OF THE STUDY: This should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.
AIMS: This paper reports a simple, rapid approach for the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC). METHODS AND RESULTS: Direct PCR (DPCR) obviates the need for the recovery of cells from the sample or DNA extraction prior to PCR. Primers specific for Stx-encoding genes stx1 and stx2 were used in DPCR for the detection of E. coli O157:H7 added to environmental water samples and milk. CONCLUSIONS: PCR reactions containing one cell yielded a DPCR product. SIGNIFICANCE AND IMPACT OF THE STUDY: This should provide an improved method to assess contamination of environmental and other samples by STEC and other pathogens.
Authors: Susana Campuzano; Jahir Orozco; Daniel Kagan; Maria Guix; Wei Gao; Sirilak Sattayasamitsathit; Jonathan C Claussen; Arben Merkoçi; Joseph Wang Journal: Nano Lett Date: 2011-12-07 Impact factor: 11.189
Authors: Nawal Nouridaim Abuelhassan; Sahilah Abdul Mutalib; Fufa Ido Gimba; Wan Mohtar Yusoff Journal: Environ Sci Pollut Res Int Date: 2016-05-27 Impact factor: 4.223