F Al-Mulla1, M Al-Maghrebi, G Varadharaj. 1. Department of Pathology, Molecular Pathology Unit, Kuwait University, Faculty of Medicine, PO Box 24923, Safat, Kuwait 13110. almulla@hsc.kuniv.edu.kw
Abstract
AIMS: To describe a cytogenetic technique suitable for the rapid assessment of global gene expression that is based on comparative genomic hybridisation (CGH), and to use it to understand the relation between genetic amplifications and gene expression. METHODS: Whereas traditional CGH uses DNA as test and reference in hybridisations, expressive genomic hybridisation (EGH) uses globally amplified mRNA as test and normal DNA as reference. EGH is a rapid and powerful tool for localising and studying global gene expression profiles and correlating them with loci of genetic amplifications using traditional CGH. RESULTS: EGH was used to correlate genetic amplifications detected by CGH with the expression profile of two independent cell lines-Colo320 and T47D. Although many amplifications resulted in overexpression, other amplifications were partially or completely silenced at the cytogenetic level. CONCLUSION: This technique will assist in the analysis of overexpressed genes within amplicons and could resolve a controversial issue in cancer cytogenetics; namely, the relation between genetic amplifications and overexpression.
AIMS: To describe a cytogenetic technique suitable for the rapid assessment of global gene expression that is based on comparative genomic hybridisation (CGH), and to use it to understand the relation between genetic amplifications and gene expression. METHODS: Whereas traditional CGH uses DNA as test and reference in hybridisations, expressive genomic hybridisation (EGH) uses globally amplified mRNA as test and normal DNA as reference. EGH is a rapid and powerful tool for localising and studying global gene expression profiles and correlating them with loci of genetic amplifications using traditional CGH. RESULTS: EGH was used to correlate genetic amplifications detected by CGH with the expression profile of two independent cell lines-Colo320 and T47D. Although many amplifications resulted in overexpression, other amplifications were partially or completely silenced at the cytogenetic level. CONCLUSION: This technique will assist in the analysis of overexpressed genes within amplicons and could resolve a controversial issue in cancer cytogenetics; namely, the relation between genetic amplifications and overexpression.
Authors: A M Snijders; N Nowak; R Segraves; S Blackwood; N Brown; J Conroy; G Hamilton; A K Hindle; B Huey; K Kimura; S Law; K Myambo; J Palmer; B Ylstra; J P Yue; J W Gray; A N Jain; D Pinkel; D G Albertson Journal: Nat Genet Date: 2001-11 Impact factor: 38.330
Authors: Swen Wessendorf; Björn Fritz; Gunnar Wrobel; Michelle Nessling; Stefan Lampel; Daniel Göettel; Manfred Küepper; Stefan Joos; Ton Hopman; Felix Kokocinski; Hartmut Döhner; Martin Bentz; Carsten Schwäenen; Peter Lichter Journal: Lab Invest Date: 2002-01 Impact factor: 5.662
Authors: Y J Lu; D Williamson; J Clark; R Wang; N Tiffin; L Skelton; T Gordon; R Williams; B Allan; A Jackman; C Cooper; K Pritchard-Jones; J Shipley Journal: Proc Natl Acad Sci U S A Date: 2001-07-31 Impact factor: 11.205
Authors: F Forozan; E H Mahlamäki; O Monni; Y Chen; R Veldman; Y Jiang; G C Gooden; S P Ethier; A Kallioniemi; O P Kallioniemi Journal: Cancer Res Date: 2000-08-15 Impact factor: 12.701
Authors: O Monni; M Barlund; S Mousses; J Kononen; G Sauter; M Heiskanen; P Paavola; K Avela; Y Chen; M L Bittner; A Kallioniemi Journal: Proc Natl Acad Sci U S A Date: 2001-05-01 Impact factor: 11.205
Authors: C Sakakura; A Hagiwara; R Yasuoka; Y Fujita; M Nakanishi; K Masuda; A Kimura; Y Nakamura; J Inazawa; T Abe; H Yamagishi Journal: Int J Cancer Date: 2000-05-20 Impact factor: 7.396