Literature DB >> 12860714

rRNA intergenic spacer regions for phylogenetic analysis of Rickettsia species.

Liliana Vitorino1, Líbia Zé-Zé, Ana Sousa, Fatima Bacellar, Rogério Tenreiro.   

Abstract

Species of the genus Rickettsia are responsible for several human diseases, namely epidemic typhus, Rocky Mountain spotted fever, and tick-borne typhus transmitted by arthropod vectors. The rrl-rrf intergenic spacer region (rrl-rrf ITS) was sequenced for 12 Rickettsia strains, including R. typhi, 6 untested species, R. aeschlimannii, Bar29, R. helvetica, R. honei, R. massilae, and R. slovaca as well as 5 Portuguese isolates. Phylogenetic trees inferred from rrl-rrf spacer sequences using maximum-parsimony and distance methods provided largely congruent tree topologies, supported by significant bootstrap values, enabling the identification of five distinct rickettsiae clusters.

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Year:  2003        PMID: 12860714     DOI: 10.1111/j.1749-6632.2003.tb07451.x

Source DB:  PubMed          Journal:  Ann N Y Acad Sci        ISSN: 0077-8923            Impact factor:   5.691


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