| Literature DB >> 12837249 |
Wen Tao Peng1, Mark D Robinson, Sanie Mnaimneh, Nevan J Krogan, Gerard Cagney, Quaid Morris, Armaity P Davierwala, Jörg Grigull, Xueqi Yang, Wen Zhang, Nicholas Mitsakakis, Owen W Ryan, Nira Datta, Vladimir Jojic, Chris Pal, Veronica Canadien, Dawn Richards, Bryan Beattie, Lani F Wu, Steven J Altschuler, Sam Roweis, Brendan J Frey, Andrew Emili, Jack F Greenblatt, Timothy R Hughes.
Abstract
Predictive analysis using publicly available yeast functional genomics and proteomics data suggests that many more proteins may be involved in biogenesis of ribonucleoproteins than are currently known. Using a microarray that monitors abundance and processing of noncoding RNAs, we analyzed 468 yeast strains carrying mutations in protein-coding genes, most of which have not previously been associated with RNA or RNP synthesis. Many strains mutated in uncharacterized genes displayed aberrant noncoding RNA profiles. Ten factors involved in noncoding RNA biogenesis were verified by further experimentation, including a protein required for 20S pre-rRNA processing (Tsr2p), a protein associated with the nuclear exosome (Lrp1p), and a factor required for box C/D snoRNA accumulation (Bcd1p). These data present a global view of yeast noncoding RNA processing and confirm that many currently uncharacterized yeast proteins are involved in biogenesis of noncoding RNA.Entities:
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Year: 2003 PMID： 12837249 DOI： 10.1016/s0092-8674(03)00466-5
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582