Literature DB >> 12791864

Evaluation of repetitive element sequence-based PCR as a molecular typing method for Clostridium difficile.

Patrizia Spigaglia1, Paola Mastrantonio.   

Abstract

Repetitive element sequence-based PCR (rep-PCR) is a typing method that enables the generation of DNA fingerprinting that discriminates bacterial strains. In this study, we evaluated the applicability of rep-PCR in typing Clostridium difficile clinical isolates. The results obtained by rep-PCR were compared with those obtained by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping. A high correspondence between pattern differentiations produced by rep-PCR and PFGE was observed, whereas PCR ribotyping showed a lower level of discriminatory power.

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Year:  2003        PMID： 12791864      PMCID： PMC156551          DOI： 10.1128/JCM.41.6.2454-2457.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  25 in total

1.  Evaluation of methods for molecular typing and identification of members of the genus Brevibacterium and other related species.

Authors:  Artur Alves; Orlando Santos; Isabel Henriques; António Correia
Journal:  FEMS Microbiol Lett       Date:  2002-08-06       Impact factor: 2.742

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Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

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Journal:  Mol Microbiol       Date:  1991-04       Impact factor: 3.501

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Journal:  Cell       Date:  1984-07       Impact factor: 41.582

9.  Molecular typing and long-term comparison of clostridium difficile strains by pulsed-field gel electrophoresis and PCR-ribotyping.

Authors:  P Spigaglia; R Cardines; S Rossi; M G Menozzi; P Mastrantonio
Journal:  J Med Microbiol       Date:  2001-05       Impact factor: 2.472

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Authors:  J Versalovic; T Koeuth; J R Lupski
Journal:  Nucleic Acids Res       Date:  1991-12-25       Impact factor: 16.971
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  8 in total

1.  Molecular characterization of hand flora and environmental isolates in a community setting.

Authors:  Preeti Pancholi; Mimi Healy; Tracy Bittner; Renee Webb; Fan Wu; Allison Aiello; Elaine Larson; Phyllis Della Latta
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

2.  Comparison of a commercially available repetitive-element PCR system (DiversiLab) with PCR ribotyping for typing of clostridium difficile strains.

Authors:  C Eckert; J Van Broeck; P Spigaglia; B Burghoffer; M Delmée; P Mastrantonio; F Barbut
Journal:  J Clin Microbiol       Date:  2011-07-20       Impact factor: 5.948

3.  Clustering of clinical and environmental Escherichia coli O104 isolates using the DiversiLab™ repetitive sequence-based PCR system.

Authors:  N M Herbold; L M Clotilde; K M Anderson; J Kase; G L Hartman; S Himathongkham; A Lin; C R Lauzon
Journal:  Curr Microbiol       Date:  2014-12-02       Impact factor: 2.188

4.  Fingerprinting of Bacillus thuringiensis type strains and isolates by using Bacillus cereus group-specific repetitive extragenic palindromic sequence-based PCR analysis.

Authors:  Arturo Reyes-Ramirez; Jorge E Ibarra
Journal:  Appl Environ Microbiol       Date:  2005-03       Impact factor: 4.792

5.  Molecular subtyping of poultry-associated type A Clostridium perfringens isolates by repetitive-element PCR.

Authors:  G R Siragusa; M D Danyluk; K L Hiett; M G Wise; S E Craven
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

6.  Molecular analysis of Clostridium difficile at a university teaching hospital in Japan: a shift in the predominant type over a five-year period.

Authors:  E Sawabe; H Kato; K Osawa; T Chida; N Tojo; Y Arakawa; N Okamura
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-10       Impact factor: 3.267

Review 7.  Clostridium difficile bacteremia, Taiwan.

Authors:  Nan-Yao Lee; Yu-Tsung Huang; Po-Ren Hsueh; Wen-Chien Ko
Journal:  Emerg Infect Dis       Date:  2010-08       Impact factor: 6.883

8.  A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST.

Authors:  Roberto A Viau; Lee M Kiedrowski; Barry N Kreiswirth; Mark Adams; Federico Perez; Dror Marchaim; Dubert M Guerrero; Keith S Kaye; Latania K Logan; Maria Virginia Villegas; Robert A Bonomo
Journal:  Pathog Immun       Date:  2017-02-01
  8 in total

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