Literature DB >> 21775548

Comparison of a commercially available repetitive-element PCR system (DiversiLab) with PCR ribotyping for typing of clostridium difficile strains.

C Eckert1, J Van Broeck, P Spigaglia, B Burghoffer, M Delmée, P Mastrantonio, F Barbut.   

Abstract

This study compared a repetitive-element PCR (rep-PCR) method (DiversiLab system) to PCR ribotyping. The discriminatory power of rep-PCR was 0.997. Among the PCR ribotype 027 isolates tested, different rep types could be distinguished. rep-PCR showed a higher discriminatory power than PCR ribotyping. Nevertheless, this method requires technical skill, and visual interpretation of rep-PCR fingerprint patterns may be difficult.

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Year:  2011        PMID： 21775548      PMCID： PMC3165626          DOI： 10.1128/JCM.00324-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

1.  Stability of repetitive-sequence PCR patterns with respect to culture age and subculture frequency.

Authors:  Hyunseok Peter Kang; W Michael Dunne
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

2.  Evaluation of repetitive element sequence-based PCR as a molecular typing method for Clostridium difficile.

Authors:  Patrizia Spigaglia; Paola Mastrantonio
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

3.  Comparison of repetitive extragenic palindromic sequence-based PCR with PCR ribotyping and pulsed-field gel electrophoresis in studying the clonality of Clostridium difficile.

Authors:  T Pasanen; S M Kotila; J Horsma; A Virolainen; J Jalava; S Ibrahem; J Antikainen; S Mero; E Tarkka; M Vaara; P Tissari
Journal:  Clin Microbiol Infect       Date:  2011-02       Impact factor: 8.067

4.  Microbial DNA typing by automated repetitive-sequence-based PCR.

Authors:  Mimi Healy; Joe Huong; Traci Bittner; Maricel Lising; Stacie Frye; Sabeen Raza; Robert Schrock; Janet Manry; Alex Renwick; Robert Nieto; Charles Woods; James Versalovic; James R Lupski
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

5.  PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes.

Authors:  S L Stubbs; J S Brazier; G L O'Neill; B I Duerden
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

6.  Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.

Authors:  P R Hunter; M A Gaston
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

7.  Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

Authors:  P Bidet; V Lalande; B Salauze; B Burghoffer; V Avesani; M Delmée; A Rossier; F Barbut; J C Petit
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

8.  Antimicrobial susceptibilities and molecular epidemiology of clinical isolates of Clostridium difficile in taiwan.

Authors:  Yi-Chun Lin; Yu-Tsung Huang; Pei-Jane Tsai; Tai-Fen Lee; Nan-Yao Lee; Chun-Hsing Liao; Shyr-Yi Lin; Wen-Chien Ko; Po-Ren Hsueh
Journal:  Antimicrob Agents Chemother       Date:  2011-01-24       Impact factor: 5.191

9.  Prospective study of Clostridium difficile infections in Europe with phenotypic and genotypic characterisation of the isolates.

Authors:  F Barbut; P Mastrantonio; M Delmée; J Brazier; E Kuijper; I Poxton
Journal:  Clin Microbiol Infect       Date:  2007-09-11       Impact factor: 8.067

10.  Comparison of seven techniques for typing international epidemic strains of Clostridium difficile: restriction endonuclease analysis, pulsed-field gel electrophoresis, PCR-ribotyping, multilocus sequence typing, multilocus variable-number tandem-repeat analysis, amplified fragment length polymorphism, and surface layer protein A gene sequence typing.

Authors:  George Killgore; Angela Thompson; Stuart Johnson; Jon Brazier; Ed Kuijper; Jacques Pepin; Eric H Frost; Paul Savelkoul; Brad Nicholson; Renate J van den Berg; Haru Kato; Susan P Sambol; Walter Zukowski; Christopher Woods; Brandi Limbago; Dale N Gerding; L Clifford McDonald
Journal:  J Clin Microbiol       Date:  2007-11-26       Impact factor: 5.948
  10 in total
  2 in total

Review 1.  Challenges for standardization of Clostridium difficile typing methods.

Authors:  Charlotte A Huber; Niki F Foster; Thomas V Riley; David L Paterson
Journal:  J Clin Microbiol       Date:  2013-06-19       Impact factor: 5.948

2.  Development and validation of an internationally-standardized, high-resolution capillary gel-based electrophoresis PCR-ribotyping protocol for Clostridium difficile.

Authors:  Warren N Fawley; C W Knetsch; Duncan R MacCannell; Celine Harmanus; Tim Du; Michael R Mulvey; Ashley Paulick; Lydia Anderson; E J Kuijper; Mark H Wilcox
Journal:  PLoS One       Date:  2015-02-13       Impact factor: 3.240
  2 in total

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