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Literature DB >> 12746558

A novel method for preparing single-stranded DNA for pyrosequencing.

Abstract

Pyrosequencing technology is a powerful genotyping tool that requires the generation of single stranded DNA. Currently, two simple, solid-phase-based methods are available for this, but they require special equipment, they are not automated, and they are relatively expensive because of the need for biotinylated polymerase chain reaction primers. In this article, an enzymatic liquid-phase method for the generation of high-quality, single-stranded DNA, and its novel use for Pyrosequencing are described. The method has also been fully automated.

Mesh：

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Year: 2003 PMID： 12746558 DOI： 10.1385/mb:24:2:221

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

9 in total

Review 1. Pyrosequencing sheds light on DNA sequencing.

Authors: M Ronaghi
Journal: Genome Res Date: 2001-01 Impact factor: 9.043

Review 2. Molecular techniques: divide or share.

Authors: M Boxer
Journal: J Clin Pathol Date: 2000-01 Impact factor: 3.411

3. Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene.

Authors: H Unnerstad; H Ericsson; A Alderborn; W Tham; M L Danielsson-Tham; J G Mattsson
Journal: Appl Environ Microbiol Date: 2001-11 Impact factor: 4.792

Review 4. Principles and applications of methods for DNA-based typing of microbial organisms.

Authors: D M Olive; P Bean
Journal: J Clin Microbiol Date: 1999-06 Impact factor: 5.948

5. Rapid molecular identification and subtyping of Helicobacter pylori by pyrosequencing of the 16S rDNA variable V1 and V3 regions.

Authors: H Monstein; S Nikpour-Badr; J Jonasson
Journal: FEMS Microbiol Lett Date: 2001-05-15 Impact factor: 2.742

6. What a load of old sequence!!!

Authors: Mathew A Diggle; Stuart C Clarke
Journal: J Clin Microbiol Date: 2002-07 Impact factor: 5.948

Review 7. Nucleotide sequence-based typing of bacteria and the impact of automation.

Authors: Stuart C Clarke
Journal: Bioessays Date: 2002-09 Impact factor: 4.345

8. Automated PCR/sequence template purification.

Authors: S C Clarke; M A Diggle
Journal: Mol Biotechnol Date: 2002-07 Impact factor: 2.695

9. Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis.

Authors: S C Clarke; M A Diggle; G F Edwards
Journal: J Clin Microbiol Date: 2001-09 Impact factor: 5.948

9 in total

6 in total

1. Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci.

Authors: Alistair Sinclair; Catherine Arnold; Neil Woodford
Journal: Antimicrob Agents Chemother Date: 2003-11 Impact factor: 5.191

A novel method for preparing single-stranded DNA for pyrosequencing.

Review 1. Pyrosequencing sheds light on DNA sequencing.

Review 2. Molecular techniques: divide or share.

3. Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene.

Review 4. Principles and applications of methods for DNA-based typing of microbial organisms.

5. Rapid molecular identification and subtyping of Helicobacter pylori by pyrosequencing of the 16S rDNA variable V1 and V3 regions.

6. What a load of old sequence!!!

Review 7. Nucleotide sequence-based typing of bacteria and the impact of automation.

8. Automated PCR/sequence template purification.

9. Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis.

1. Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci.

Review 2. Pyrosequencing: sequence typing at the speed of light.

Review 3. Genotypic characterization of Neisseria meningitidis using Pyrosequencing.

4. Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR.

5. Methods for the preparation of large quantities of complex single-stranded oligonucleotide libraries.

6. On-Flow Synthesis of Co-Polymerizable Oligo-Microspheres and Application in ssDNA Amplification.