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Literature DB >> 12102546

Automated PCR/sequence template purification.

Abstract

A commercially available filtration method is described for the purification of polymerase chain reaction (PCR) templates and sequence-labeled products. The methodology is described for the automation of this application and its use on a high-throughput liquid handling robot and capillary-based automated DNA sequencer. The application provides good-quality DNA, is relatively cheap, and can be used in either 96- or 384-well format.

Mesh：

Year: 2002 PMID： 12102546 DOI： 10.1385/MB:21:3:221

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

14 in total

Review 1. Molecular techniques: divide or share.

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Review 2. Molecular bacteriology: a diagnostic tool for the millennium.

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Review 3. From microbial genome sequence to applications.

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4. Isolation of genomic DNA from blood using a novel filter-based DNA purification technology.

Authors: M P Baker; A Mitchell; C Bridge; S Martin; F Holder; N D Pathirana; A Jarvis; N Butt
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Review 5. Principles and applications of methods for DNA-based typing of microbial organisms.

Authors: D M Olive; P Bean
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6. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms.

Authors: M C Maiden; J A Bygraves; E Feil; G Morelli; J E Russell; R Urwin; Q Zhang; J Zhou; K Zurth; D A Caugant; I M Feavers; M Achtman; B G Spratt
Journal: Proc Natl Acad Sci U S A Date: 1998-03-17 Impact factor: 11.205

7. Method for 96-well M13 DNA template preparations for large-scale sequencing.

Authors: B Andersson; J Lu; K E Edwards; D M Muzny; R A Gibbs
Journal: Biotechniques Date: 1996-06 Impact factor: 1.993

8. Semiautomation of multilocus sequence typing for the characterization of clinical isolates of Neisseria meningitidis.

Authors: S C Clarke; M A Diggle; G F Edwards
Journal: J Clin Microbiol Date: 2001-09 Impact factor: 5.948

9. Introduction of an automated service for the laboratory confirmation of meningococcal disease in Scotland.

Authors: S C Clarke; M A Diggle; J A Reid; L Thom; G F Edwards
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10. A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease.

Authors: Mark C Enright; Brian G Spratt
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6 in total

1. A novel method for preparing single-stranded DNA for pyrosequencing.

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Journal: Mol Biotechnol Date: 2003-06 Impact factor: 2.695

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3. Transforming microbial genotyping: a robotic pipeline for genotyping bacterial strains.

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4. Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer.

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5. Simultaneous nasopharyngeal carriage of two pneumococcal multilocus sequence types with a serotype 3 phenotype.

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6. Death or survival from invasive pneumococcal disease in Scotland: associations with serogroups and multilocus sequence types.

Authors: Donald Inverarity; Karen Lamb; Mathew Diggle; Chris Robertson; David Greenhalgh; Tim J Mitchell; Andrew Smith; Johanna M C Jefferies; Stuart C Clarke; Jim McMenamin; Giles F S Edwards
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6 in total