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Literature DB >> 1272805

Interpretation of the properties of chromatin extracts from mammalian nuclei.

Abstract

Chromatin from standard preparations of nuclei stabilized by magnesium ions at 0-4 degrees C was degraded during the nuclear isolation, and newly-synthesized chromatin was degraded slightly more slowly than the ;older' chromatin. The significance of this observation, and its relation to the interpretation of the properties of nucleoprotein extracts is discussed.

Entities: Chemical Species

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Year: 1976 PMID： 1272805 PMCID： PMC342969 DOI： 10.1093/nar/3.4.1101

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. Fractionation of Chromatin by thermal precipitation in phosphate buffer.

Authors: G G Markov; I G Ivanov; I G Pashev
Journal: J Mol Biol Date: 1975-04-25 Impact factor: 5.469

2. Nuclear protein matrix: association with newly synthesized DNA.

Authors: R Berezney; D S Coffey
Journal: Science Date: 1975-07-25 Impact factor: 47.728

3. Preparation of native chromatin and damage caused by shearing.

Authors: M Noll; J O Thomas; R D Kornberg
Journal: Science Date: 1975-03-28 Impact factor: 47.728

4. The calcium dependent endonuclease activity of isolated nuclear preparations. Relationships between its occurrence and the occurrence of other classes of enzymes found in nuclear preparations.

Authors: D R Hewish; L A Burgoyne
Journal: Biochem Biophys Res Commun Date: 1973-05-15 Impact factor: 3.575

5. Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis.

Authors: L A Burgoyne; D R Hewish; J Mobbs
Journal: Biochem J Date: 1974-10 Impact factor: 3.857

6. Partial purification of the template-active fraction of chromatin: a preliminary report.

Authors: J M Gottesfeld; W T Garrard; G Bagi; R F Wilson; J Bonner
Journal: Proc Natl Acad Sci U S A Date: 1974-06 Impact factor: 11.205

7. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.

Authors: D R Hewish; L A Burgoyne
Journal: Biochem Biophys Res Commun Date: 1973-05-15 Impact factor: 3.575

8. Incorporation of radioactivity from thymidine into mammalian glucose and glycogen.

Authors: R L Dobson; M F Cooper
Journal: Biochim Biophys Acta Date: 1971-12-30

9. A new type of deoxyribonucleoprotein isolated from rabbit thymus nuclei.

Authors: A W Rees; R C Krueger
Journal: Biochim Biophys Acta Date: 1968-07-23

10. Increased thermal stability of chromatin containing 5-bromodeoxyuridine-substituted DNA.

Authors: J David; J S Gordon; W J Rutter
Journal: Proc Natl Acad Sci U S A Date: 1974-07 Impact factor: 11.205

18 in total

1. Chromatin structure: a property of the higher structures of chromatin and in the time course of its formation during chromatin replication.

Authors: L A Burgoyne; J D Mobbs; A J Marshall
Journal: Nucleic Acids Res Date: 1976-12 Impact factor: 16.971

Interpretation of the properties of chromatin extracts from mammalian nuclei.

1. Fractionation of Chromatin by thermal precipitation in phosphate buffer.

2. Nuclear protein matrix: association with newly synthesized DNA.

3. Preparation of native chromatin and damage caused by shearing.

4. The calcium dependent endonuclease activity of isolated nuclear preparations. Relationships between its occurrence and the occurrence of other classes of enzymes found in nuclear preparations.

5. Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis.

6. Partial purification of the template-active fraction of chromatin: a preliminary report.

7. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.

8. Incorporation of radioactivity from thymidine into mammalian glucose and glycogen.

9. A new type of deoxyribonucleoprotein isolated from rabbit thymus nuclei.

10. Increased thermal stability of chromatin containing 5-bromodeoxyuridine-substituted DNA.

1. Chromatin structure: a property of the higher structures of chromatin and in the time course of its formation during chromatin replication.

2. Nucleotide sequence of the chicken 5-aminolevulinate synthase gene.

3. A genetic locus closely linked to a protease inhibitor gene complex controls the level of multiple RNA transcripts.

4. DNA degradation in terminally differentiating lens fiber cells from chick embryos.

5. Intragenic DNA spacers interrupt the ovalbumin gene.

6. Use of linkage disequilibrium data in prenatal diagnosis of cystic fibrosis.

7. Confirmation of prenatal diagnosis of cystic fibrosis by DNA typing of fetal tissues.

8. Reconstitution of a deoxyribonuclease I-sensitive structure on active genes.

9. Isolation of newly replicated chromatin by using shallow metrizamide gradients.

10. Interaction of oestrogen and progesterone receptors with specific subfractions of laying-hen oviduct chromatin.