Literature DB >> 4526205

Partial purification of the template-active fraction of chromatin: a preliminary report.

J M Gottesfeld, W T Garrard, G Bagi, R F Wilson, J Bonner.   

Abstract

A fraction of rat-liver chromatin that is transcriptionally active in vivo has been purified 6- to 7-fold over whole chromatin. This was accomplished by selectively shearing chromatin with DNase II followed by fractionating the released portion on the basis of its solubility properties in 2 mM MgCl(2). The resulting soluble material comprises 11% of the total chromatin DNA and is impoverished in histone and enriched in nonhistone protein. Compared with unsheared chromatin, this minor fraction exhibits marked differences in chromosomal protein species. DNA renaturation studies indicate that this fraction is composed of a specific subset of whole genomal DNA sequences. Furthermore, DNA.RNA hybridization experiments suggest that almost 60% of the nonrepetitious DNA sequences of this minor fraction could code for cellular RNA.

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Year:  1974        PMID： 4526205      PMCID： PMC388417          DOI： 10.1073/pnas.71.6.2193

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  17 in total

1.  Preparation of highly purified ribonucleic acid polymerase; separation from polynucleotide phosphorylase and polyphosphate kinase.

Authors:  D J McConnell; J Bonner
Journal:  Biochemistry       Date:  1972-11-07       Impact factor: 3.162

2.  Studies on the nuclear 4 to 7 s RNA of the Novikoff hepatoma.

Authors:  Y Moriyama; J L Hodnett; A W Prestayko; H Busch
Journal:  J Mol Biol       Date:  1969-01       Impact factor: 5.469

3.  Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro.

Authors:  R Axel; H Cedar; G Felsenfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1973-07       Impact factor: 11.205

4.  Iodination of nucleic acids in vitro.

Authors:  S L Commerford
Journal:  Biochemistry       Date:  1971-05-25       Impact factor: 3.162

5.  Transcription of nonrepeated DNA during mouse and rabbit development.

Authors:  I R Brown; R B Church
Journal:  Dev Biol       Date:  1972-09       Impact factor: 3.582

6.  The structure of chromatin as revealed by deoxyribonuclease digestion studies.

Authors:  R J Billing; J Bonner
Journal:  Biochim Biophys Acta       Date:  1972-10-27

7.  Fractionation of chromatin by thermal chromatography.

Authors:  B L McConaughy; B J McCarthy
Journal:  Biochemistry       Date:  1972-03-14       Impact factor: 3.162

8.  Repetitive and non-repetitive DNA sequences and a speculation on the origins of evolutionary novelty.

Authors:  R J Britten; E H Davidson
Journal:  Q Rev Biol       Date:  1971-06       Impact factor: 4.875

9.  Fractionation of liver chromatin.

Authors:  K Marushige; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1971-12       Impact factor: 11.205

10.  High resolution acrylamide gel electrophoresis of histones.

Authors:  S Panyim; R Chalkley
Journal:  Arch Biochem Biophys       Date:  1969-03       Impact factor: 4.013
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  52 in total

1.  Activation of chromatin by acetylation of histone side chains.

Authors:  K Marushige
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

2.  Major nonhistone proteins of rat liver chromatin: preliminary identification of myosin, actin, tubulin, and tropomyosin.

Authors:  A S Douvas; C A Harrington; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

3.  Structure of transcriptionally active chromatin.

Authors:  J M Gottesfeld; R F Murphy; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

4.  Chemical and physical properties of fractionated chromatin.

Authors:  E M Berkowitz; P Doty
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

5.  Interpretation of the properties of chromatin extracts from mammalian nuclei.

Authors:  A J Marshall; L A Burgoyne
Journal:  Nucleic Acids Res       Date:  1976-04       Impact factor: 16.971

6.  Structure of transcriptionally-active chromatin subunits.

Authors:  J M Gottesfeld; P J Butler
Journal:  Nucleic Acids Res       Date:  1977-09       Impact factor: 16.971

7.  The absence of histone H1 from the chromatin fraction obtained by sonication of calf thymus nuclei under "quasiphysiological" ionic conditions.

Authors:  A I Lishanskaya; M I Mosevitsky
Journal:  Nucleic Acids Res       Date:  1976-08       Impact factor: 16.971

8.  Isolation and physicochemical characterisation of the DNA from normal human skin and psoriatic scales.

Authors:  G Teimer; G Ramb; G Leonhardi
Journal:  Arch Dermatol Res       Date:  1976-10-27       Impact factor: 3.017

9.  Histone content in relation to amount of heterochromatin and developmental stage in three species of Drosophila.

Authors:  P Holmgren; B Rasmuson; T Johansson; G Sundquist
Journal:  Chromosoma       Date:  1976-02-13       Impact factor: 4.316

10.  Tetrahymena ribosomal RNA gene chromatin is digested by micrococcal nuclease at sites which have the same regular spacing on the DNA as corresponding sites in the bulk nuclear chromatin.

Authors:  P W Piper; J Celis; K Kaltoft; J C Leer; O F Nielsen; O Westergaard
Journal:  Nucleic Acids Res       Date:  1976-02       Impact factor: 16.971
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