Literature DB >> 1257052

Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide.

P T Lomedico, G F Saunders.   

Abstract

Total nucleic acid extraction and selection of poly A-containing molecules yield preparative quantities of undegraded mRNA from adult and fetal pancreas. Using a stringent immunoassay, this mRNA is found to direct the synthesis of an immunoreactive insulin polypeptide in the wheat germ translation system. On sodium dodecyl sulfate polyacrylamide gels, this polypeptide (12,000-13,000 daltons) is larger than proinsulin (9,000 daltons).

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Year:  1976        PMID： 1257052      PMCID： PMC342908          DOI： 10.1093/nar/3.2.381

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  26 in total

1.  The translation of a human placental lactogen mRNA fraction in heterologous cell-free systems: the synthesis of a possible precursor.

Authors:  I Boime; S Boguslawski; J Caine
Journal:  Biochem Biophys Res Commun       Date:  1975-01-06       Impact factor: 3.575

2.  Tobacco mosaic virus RNA directs the synthesis of a coat protein peptide in a cell-free system from wheat.

Authors:  B E Roberts
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469

3.  Translation of satellite tobacco necrosis virus ribonucleic acid. I. Characterization of in vitro procaryotic and eucaryotic translation products.

Authors:  W H Klein; C Nolan; J M Lazar; J M Clark
Journal:  Biochemistry       Date:  1972-05-23       Impact factor: 3.162

4.  Cell-free translation of messenger RNA of simian virus 40: synthesis of the major capsid protein.

Authors:  C L Prives; H Aviv; B M Paterson; B E Roberts; S Rozenblatt; M Revel; E Winocour
Journal:  Proc Natl Acad Sci U S A       Date:  1974-02       Impact factor: 11.205

5.  Pre-proparathyroid hormone: a direct translation product of parathyroid messenger RNA.

Authors:  B Kemper; J F Habener; R C Mulligan; J T Potts; A Rich
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205

6.  A possible precursor of immunoglobulin light chains.

Authors:  C Milstein; G G Brownlee; T M Harrison; M B Mathews
Journal:  Nat New Biol       Date:  1972-09-27

7.  Purification of 14S messenger RNA of immunoglobulin light chain that codes for a possible light-chain precursor.

Authors:  B Mach; C Faust; P Vassalli
Journal:  Proc Natl Acad Sci U S A       Date:  1973-02       Impact factor: 11.205

8.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

9.  Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine.

Authors:  I Schechter
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205

10.  Determination of actin messenger RNA in cultures of differentiating embryonic chick skeletal muscle.

Authors:  B M Paterson; B E Roberts; D Yaffe
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205
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  27 in total

1.  In vitro synthesis of pre-proteins of vacuolar compartmented proteinase inhibitors that accumulate in leaves of wounded tomato plants.

Authors:  C E Nelson; C A Ryan
Journal:  Proc Natl Acad Sci U S A       Date:  1980-04       Impact factor: 11.205

2.  Partial purification and characterization of the mRNA for rat preproinsulin.

Authors:  J R Duguid; D F Steiner; W L Chick
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

3.  Detection and kinetic behavior of preproinsulin in pancreatic islets.

Authors:  C Patzelt; A D Labrecque; J R Duguid; R J Carroll; P S Keim; R L Heinrikson; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

4.  The primary structure of bovine monoamine oxidase type A. Comparison with peptide sequences of bovine monoamine oxidase type B and other flavoenzymes.

Authors:  J F Powell; Y P Hsu; W Weyler; S A Chen; J Salach; K Andrikopoulos; J Mallet; X O Breakefield
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

5.  A bacterial clone synthesizing proinsulin.

Authors:  L Villa-Komaroff; A Efstratiadis; S Broome; P Lomedico; R Tizard; S P Naber; W L Chick; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

6.  Complete coding sequence of rat tyrosine hydroxylase mRNA.

Authors:  B Grima; A Lamouroux; F Blanot; N F Biguet; J Mallet
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

7.  Antibodies of the secondary response can be expressed without switch recombination in normal mouse B cells.

Authors:  A P Perlmutter; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-11       Impact factor: 11.205

8.  Localization of the human insulin gene to the distal end of the short arm of chromosome 11.

Authors:  M E Harper; A Ullrich; G F Saunders
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205

9.  Adaptative decrease in expression of the mRNA for uncoupling protein and subunit II of cytochrome c oxidase in rat brown adipose tissue during pregnancy and lactation.

Authors:  I Martin; M Giralt; O Viñas; R Iglesias; T Mampel; F Villarroya
Journal:  Biochem J       Date:  1989-11-01       Impact factor: 3.857

10.  Characterization of proinsulin-insulin intermediates in human plasma.

Authors:  C de Haën; S A Little; J M May; R H Williams
Journal:  J Clin Invest       Date:  1978-10       Impact factor: 14.808
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