Literature DB >> 185618

Partial purification and characterization of the mRNA for rat preproinsulin.

J R Duguid, D F Steiner, W L Chick.   

Abstract

Electrophoretically homogeneous messenger RNA for rat preproinsulin has been prepared from an islet cell tumor by the use of oligo(dT(-cellulose chromatography and sucrose density gradient centrifugation. The molecular weight of the mRNA is about 210,000, as determined by polyacrylamide gel electrophoresis in formamide, and its sedimentation coefficient is 9.3 S in sucrose gradients containing 0.2 M NaCl. These results indicate that the synthesis of preproinsulin is directed by a monocistronic mRNA 600 nucleotides in length and requires approximately 55% of the molecule's coding capacity.

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Year:  1976        PMID： 185618      PMCID： PMC431152          DOI： 10.1073/pnas.73.10.3539

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation.

Authors:  S Muthukrishnan; G W Both; Y Furuichi; A J Shatkin
Journal:  Nature       Date:  1975-05-01       Impact factor: 49.962

2.  Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide.

Authors:  P T Lomedico; G F Saunders
Journal:  Nucleic Acids Res       Date:  1976-02       Impact factor: 16.971

3.  The radioiodination of ribopolymers for use in hybridizational and molecular analyses.

Authors:  N H Scherberg; S Refetoff
Journal:  J Biol Chem       Date:  1974-04-10       Impact factor: 5.157

4.  Molecular weight determination of nucleic acids by gel electrophoresis in non-aqueous solution.

Authors:  D Z Staynov; J C Pinder; W B Gratzer
Journal:  Nat New Biol       Date:  1972-01-26

5.  Pancreatic islet cell tumors produced by the combined action of streptozotocin and nicotinamide.

Authors:  N Rakieten; B S Gordon; A Beaty; D A Cooney; R D Davis; P S Schein
Journal:  Proc Soc Exp Biol Med       Date:  1971-05

6.  Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate.

Authors:  R T Swank; K D Munkres
Journal:  Anal Biochem       Date:  1971-02       Impact factor: 3.365

7.  Sequence analysis of immunoglobulin light chain messenger RNA.

Authors:  C Milstein; G G Brownlee; E M Cartwright; J M Jarvis; N J Proudfoot
Journal:  Nature       Date:  1974-11-29       Impact factor: 49.962

8.  Isolation of duck haemoglobin messenger RNA and its translation by rabbit reticulocyte cell free system.

Authors:  R E Pemberton; D Housman; H F Lodish; C Baglioni
Journal:  Nat New Biol       Date:  1972-01-26

9.  Polynucleotide sequences in eukaryotic DNA and RNA that form ribonuclease-resistant complexes with polyuridylic acid.

Authors:  J O Bishop; M Rosbash
Journal:  J Mol Biol       Date:  1974-05-05       Impact factor: 5.469

10.  Isolation of a biologically active messenger RNA: preparation from fish pancreatic islets by oligo(2'-deoxythymidylic acid) affinity chromatography.

Authors:  M A Permutt; J Biesbroeck; R Chyn; I Boime; E Szczesna; D McWilliams
Journal:  Ciba Found Symp       Date:  1976
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  12 in total

1.  Identification of the major polyadenylylated transcription products and the genes active in their synthesis in a rat insulinoma.

Authors:  J R Duguid; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

2.  Cell-free synthesis of leaf protein: Identification of an apparent precursor of the small subunit of ribulose-1,5-bisphosphate carboxylase.

Authors:  A R Cashmore; M K Broadhurst; R E Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

3.  Cell-free synthesis of herpes simplex virus-coded pyrimidine deoxyribonucleoside kinase enzyme.

Authors:  C M Preston
Journal:  J Virol       Date:  1977-09       Impact factor: 5.103

4.  Detection and kinetic behavior of preproinsulin in pancreatic islets.

Authors:  C Patzelt; A D Labrecque; J R Duguid; R J Carroll; P S Keim; R L Heinrikson; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1978-03       Impact factor: 11.205

Review 5.  Regulation of proinsulin synthesis in pancreatic islets and a new aspect to insulin-dependent diabetes.

Authors:  H Okamoto
Journal:  Mol Cell Biochem       Date:  1981-06-09       Impact factor: 3.396

6.  Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs.

Authors:  Y Nabeshima; Y Fujii-Kuriyama; M Muramatsu; K Ogata
Journal:  Nucleic Acids Res       Date:  1982-10-11       Impact factor: 16.971

7.  Detection and partial sequence analysis of gastrin mRNA by using an oligodeoxynucleotide probe.

Authors:  B E Noyes; M Mevarech; R Stein; K L Agarwal
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

8.  Expression of simian virus 40-rat preproinsulin recombinants in monkey kidney cells: use of preproinsulin RNA processing signals.

Authors:  P Gruss; G Khoury
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

9.  A transplantable insulinoma in the rat.

Authors:  W L Chick; S Warren; R N Chute; A A Like; V Lauris; K C Kitchen
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

10.  Construction and selection of recombinant plasmids containing full-length complementary DNAs corresponding to rat insulins I and II.

Authors:  S J Chan; B E Noyes; K L Agarwal; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205
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