PURPOSE: To determine if careful specimen selection and washing of tissue from first trimester missed abortion products of conception specimens increases the sensitivity of routine cytogenetics in detecting aneuploidy. METHODS: Retrospective review of cytogenetics results from tissue from dilation and curettage for missed abortion in a university fertility practice between 1998 and 2001. A technique of careful selection and washing of the specimen was implemented in July 1999. Results from before (n = 15) and after (n = 41) this change were compared. Cytogenetics reports from other physicians using the same laboratory were used for comparison (n = 59). RESULTS: The percentage of 46XX results was significantly decreased in the test group when compared to historical and community controls: 29% vs. 73% and 56% respectively. The percentage of aneuploid results was significantly higher in the test group at 61% vs. 7% and 36% in the historical and community controls respectively. CONCLUSION: Thorough separation and cleaning of villi prior to sending missed abortion specimens significantly increases sensitivity of conventional cytogenetics for detecting aneuploidy by decreasing maternal contamination.
PURPOSE: To determine if careful specimen selection and washing of tissue from first trimester missed abortion products of conception specimens increases the sensitivity of routine cytogenetics in detecting aneuploidy. METHODS: Retrospective review of cytogenetics results from tissue from dilation and curettage for missed abortion in a university fertility practice between 1998 and 2001. A technique of careful selection and washing of the specimen was implemented in July 1999. Results from before (n = 15) and after (n = 41) this change were compared. Cytogenetics reports from other physicians using the same laboratory were used for comparison (n = 59). RESULTS: The percentage of 46XX results was significantly decreased in the test group when compared to historical and community controls: 29% vs. 73% and 56% respectively. The percentage of aneuploid results was significantly higher in the test group at 61% vs. 7% and 36% in the historical and community controls respectively. CONCLUSION: Thorough separation and cleaning of villi prior to sending missed abortion specimens significantly increases sensitivity of conventional cytogenetics for detecting aneuploidy by decreasing maternal contamination.
Authors: Ruth B Lathi; Jamie A M Massie; Megan Loring; Zachary P Demko; David Johnson; Styrmir Sigurjonsson; George Gemelos; Matthew Rabinowitz Journal: PLoS One Date: 2012-03-05 Impact factor: 3.240
Authors: Boris T Wang; Thomas P Chong; Fatih Z Boyar; Kimberly A Kopita; Leslie P Ross; Mohamed M El-Naggar; Trilochan Sahoo; Jia-Chi Wang; Morteza Hemmat; Mary H Haddadin; Renius Owen; Arturo L Anguiano Journal: Mol Cytogenet Date: 2014-05-22 Impact factor: 2.009