| Literature DB >> 12466305 |
Vincent VanBuren1, Yulan Piao, Dawood B Dudekula, Yong Qian, Mark G Carter, Patrick R Martin, Carole A Stagg, Uwem C Bassey, Kazuhiro Aiba, Toshio Hamatani, George J Kargul, Amber G Luo, Janet Kelso, Winston Hide, Minoru S H Ko.
Abstract
A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ~120,000 cDNA clones, which were initially re-arrayed into a set of ~11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers.Entities:
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Year: 2002 PMID： 12466305 PMCID： PMC187561 DOI： 10.1101/gr.633802
Source DB: PubMed Journal: Genome Res ISSN： 1088-9051 Impact factor: 9.043