Literature DB >> 12409416

Real-time PCR for detection and differentiation of gram-positive and gram-negative bacteria.

Sven Klaschik1, Lutz E Lehmann, Ansgar Raadts, Malte Book, Andreas Hoeft, Frank Stuber.   

Abstract

We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probes. All tested bacteria were identified correctly, and none gave a false-positive signal with the opposite Gram probe.

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Year:  2002        PMID： 12409416      PMCID： PMC139680          DOI： 10.1128/JCM.40.11.4304-4307.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

1.  Sampling variability in the microbiological evaluation of expectorated sputa and endotracheal aspirates.

Authors:  S Nagendra; P Bourbeau; S Brecher; M Dunne; M LaRocco; G Doern
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

2.  Intra and inter technologist variability in the quality assessment of respiratory tract specimens.

Authors:  G M Cooper; J J Jones; J C Arbique; G J Flowerdew; K R Forward
Journal:  Diagn Microbiol Infect Dis       Date:  2000-08       Impact factor: 2.803

3.  Use of PCR with universal primers and restriction endonuclease digestions for detection and identification of common bacterial pathogens in cerebrospinal fluid.

Authors:  J J Lu; C L Perng; S Y Lee; C C Wan
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

4.  Detection of bacterial DNA by PCR and reverse hybridization in the 16S rRNA gene with particular reference to neonatal septicemia.

Authors:  S Shang; Z Chen; X Yu
Journal:  Acta Paediatr       Date:  2001-02       Impact factor: 2.299

5.  Direct amplification of rRNA genes in diagnosis of bacterial infections.

Authors:  K Rantakokko-Jalava; S Nikkari; J Jalava; E Eerola; M Skurnik; O Meurman; O Ruuskanen; A Alanen; E Kotilainen; P Toivanen; P Kotilainen
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

6.  PCR identification of Pseudomonas aeruginosa and direct detection in clinical samples from cystic fibrosis patients.

Authors:  Luiz Vicente Ferreira DA Silva Filho; Joséa Eduardo Levi; Christina Naomi Oda Bento; Scnia Regina Testa DA Silva Ramos; Tatiana Rozov
Journal:  J Med Microbiol       Date:  1999-04       Impact factor: 2.472

7.  The use of polymerase chain reaction to detect septicemia in critically ill patients.

Authors:  R T Cursons; E Jeyerajah; J W Sleigh
Journal:  Crit Care Med       Date:  1999-05       Impact factor: 7.598

8.  Quantitative detection of Borrelia burgdorferi by real-time PCR.

Authors:  A Pahl; U Kühlbrandt; K Brune; M Röllinghoff; A Gessner
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

9.  Use of polymerase chain reaction (PCR) and DNA probe hybridization to determine the Gram reaction of the infecting bacterium in the intraocular fluids of patients with endophthalmitis.

Authors:  A R Anand; H N Madhavan; K L Therese
Journal:  J Infect       Date:  2000-11       Impact factor: 6.072

10.  Detection of and discrimination between gram-positive and gram-negative bacteria in intraocular samples by using nested PCR.

Authors:  N M Carroll; E E Jaeger; S Choudhury; A A Dunlop; M M Matheson; P Adamson; N Okhravi; S Lightman
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948
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  15 in total

1.  Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy.

Authors:  H P Horz; M E Vianna; B P F A Gomes; G Conrads
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

2.  Real-time quantitative broad-range PCR assay for detection of the 16S rRNA gene followed by sequencing for species identification.

Authors:  Franziska Zucol; Roland A Ammann; Christoph Berger; Christoph Aebi; Martin Altwegg; Felix K Niggli; David Nadal
Journal:  J Clin Microbiol       Date:  2006-08       Impact factor: 5.948

3.  Multiplexed identification of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay.

Authors:  Maneesh R Pingle; Kathleen Granger; Philip Feinberg; Rebecca Shatsky; Bram Sterling; Mark Rundell; Eric Spitzer; Davise Larone; Linnie Golightly; Francis Barany
Journal:  J Clin Microbiol       Date:  2007-04-11       Impact factor: 5.948

4.  Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.

Authors:  Sa Deepak; Kr Kottapalli; R Rakwal; G Oros; Ks Rangappa; H Iwahashi; Y Masuo; Gk Agrawal
Journal:  Curr Genomics       Date:  2007-06       Impact factor: 2.236

Review 5.  [Molecular biological detection of pathogens in patients with sepsis. Potentials, limitations and perspectives].

Authors:  K-P Hunfeld; T Bingold; V Brade; H Wissing
Journal:  Anaesthesist       Date:  2008-04       Impact factor: 1.041

6.  Detection and differentiation of in vitro-spiked bacteria by real-time PCR and melting-curve analysis.

Authors:  S Klaschik; L E Lehmann; A Raadts; M Book; J Gebel; A Hoeft; F Stuber
Journal:  J Clin Microbiol       Date:  2004-02       Impact factor: 5.948

7.  Gram stain-specific-probe-based real-time PCR for diagnosis and discrimination of bacterial neonatal sepsis.

Authors:  Yi-Dong Wu; Li-Hua Chen; Xiu-Jing Wu; Shi-Qiang Shang; Jin-Tu Lou; Li-Zhong Du; Zheng-Yan Zhao
Journal:  J Clin Microbiol       Date:  2008-06-11       Impact factor: 5.948

8.  Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis.

Authors:  Rachel K Carver-Brown; Arthur H Reis; Lisa M Rice; John W Czajka; Lawrence J Wangh
Journal:  J Pathog       Date:  2012-12-26

9.  Serum procalcitonin elevation in critically ill patients at the onset of bacteremia caused by either Gram negative or Gram positive bacteria.

Authors:  Pierre Emmanuel Charles; Sylvain Ladoire; Serge Aho; Jean-Pierre Quenot; Jean-Marc Doise; Sébastien Prin; Niels-Olivier Olsson; Bernard Blettery
Journal:  BMC Infect Dis       Date:  2008-03-26       Impact factor: 3.090

10.  Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection.

Authors:  Hideki Niimi; Tomohiro Ueno; Shirou Hayashi; Akihito Abe; Takahiro Tsurue; Masashi Mori; Homare Tabata; Hiroshi Minami; Michihiko Goto; Makoto Akiyama; Yoshihiro Yamamoto; Shigeru Saito; Isao Kitajima
Journal:  Sci Rep       Date:  2015-07-28       Impact factor: 4.379
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