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Literature DB >> 18645596

Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.

Sa Deepak¹, Kr Kottapalli, R Rakwal, G Oros, Ks Rangappa, H Iwahashi, Y Masuo, Gk Agrawal.

Abstract

Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21(st) century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.

Keywords: Real-time PCR; applications; detection; disease; microorganisms; pathogen; plant-microbe interaction; quantification

Year: 2007 PMID： 18645596 PMCID： PMC2430684 DOI： 10.2174/138920207781386960

Source DB: PubMed Journal: Curr Genomics ISSN： 1389-2029 Impact factor: 2.236

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