Literature DB >> 12409414

Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis.

Neil Woodford1, Luke Tysall, Cressida Auckland, Mark W Stockdale, Andrew J Lawson, Rachel A Walker, David M Livermore.   

Abstract

A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolates with a G2576U rRNA mutation. PCR-restriction fragment length polymorphism analysis of ribosomal DNA amplicons with NheI also detected this mutation. Both assays detected isolates heterozygous at this position. Recognition of isolates with what is presently the most frequent oxazolidinone resistance mutation may aid surveillance and individual case management.

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Year:  2002        PMID： 12409414      PMCID： PMC139696          DOI： 10.1128/JCM.40.11.4298-4300.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori.

Authors:  J R Gibson; N A Saunders; B Burke; R J Owen
Journal:  J Clin Microbiol       Date:  1999-11       Impact factor: 5.948

2.  In vitro evaluation of AZD2563, a new oxazolidinone, tested against unusual gram-positive species.

Authors:  Ronald N Jones; Douglas J Biedenbach; Tamara R Anderegg
Journal:  Diagn Microbiol Infect Dis       Date:  2002-02       Impact factor: 2.803

Review 3.  Quinupristin/dalfopristin and linezolid: where, when, which and whether to use?

Authors:  D M Livermore
Journal:  J Antimicrob Chemother       Date:  2000-09       Impact factor: 5.790

4.  Linezolid resistance in a clinical isolate of Staphylococcus aureus.

Authors:  S Tsiodras; H S Gold; G Sakoulas; G M Eliopoulos; C Wennersten; L Venkataraman; R C Moellering; M J Ferraro
Journal:  Lancet       Date:  2001-07-21       Impact factor: 79.321

5.  Resistance to linezolid: characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci.

Authors:  J Prystowsky; F Siddiqui; J Chosay; D L Shinabarger; J Millichap; L R Peterson; G A Noskin
Journal:  Antimicrob Agents Chemother       Date:  2001-07       Impact factor: 5.191

6.  Rapid identification of methicillin-resistant Staphylococcus aureus from positive blood cultures by real-time fluorescence PCR.

Authors:  T Y Tan; S Corden; R Barnes; B Cookson
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

7.  Resistance mutations in 23 S rRNA identify the site of action of the protein synthesis inhibitor linezolid in the ribosomal peptidyl transferase center.

Authors:  P Kloss; L Xiong; D L Shinabarger; A S Mankin
Journal:  J Mol Biol       Date:  1999-11-19       Impact factor: 5.469

8.  Linezolid-resistant Enterococcus faecium isolated from a patient without prior exposure to an oxazolidinone: report from the SENTRY Antimicrobial Surveillance Program.

Authors:  Ronald N Jones; Phyllis Della-Latta; Lillian V Lee; Douglas J Biedenbach
Journal:  Diagn Microbiol Infect Dis       Date:  2002-02       Impact factor: 2.803

9.  Use of a LightCycler gyrA mutation assay for rapid identification of mutations conferring decreased susceptibility to ciprofloxacin in multiresistant Salmonella enterica serotype Typhimurium DT104 isolates.

Authors:  R A Walker; N Saunders; A J Lawson; E A Lindsay; M Dassama; L R Ward; M J Woodward; R H Davies; E Liebana; E J Threlfall
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948
  9 in total
  16 in total

1.  Rapid detection and estimation by pyrosequencing of 23S rRNA genes with a single nucleotide polymorphism conferring linezolid resistance in Enterococci.

Authors:  Alistair Sinclair; Catherine Arnold; Neil Woodford
Journal:  Antimicrob Agents Chemother       Date:  2003-11       Impact factor: 5.191

2.  Molecular detection of linezolid resistance in Enterococcus faecium and Enterococcus faecalis by use of 5' nuclease real-time PCR compared to a modified classical approach.

Authors:  Guido Werner; Birgit Strommenger; Ingo Klare; Wolfgang Witte
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

Review 3.  Spread of ampicillin/vancomycin-resistant Enterococcus faecium of the epidemic-virulent clonal complex-17 carrying the genes esp and hyl in German hospitals.

Authors:  I Klare; C Konstabel; S Mueller-Bertling; G Werner; B Strommenger; C Kettlitz; S Borgmann; B Schulte; D Jonas; A Serr; A M Fahr; U Eigner; W Witte
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2005-12       Impact factor: 3.267

4.  Molecular analysis of linezolid resistance in clinical Enterococcus faecium isolates by polymerase chain reaction and pyrosequencing.

Authors:  P Schnitzler; K Schulz; C Lampson; M Geiss; H K Geiss
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2010-09-03       Impact factor: 3.267

5.  Gentamicin improves the activities of daptomycin and vancomycin against Enterococcus faecalis in vitro and in an experimental foreign-body infection model.

Authors:  Ulrika Furustrand Tafin; Ivana Majic; Cyrine Zalila Belkhodja; Bertrand Betrisey; Stéphane Corvec; Werner Zimmerli; Andrej Trampuz
Journal:  Antimicrob Agents Chemother       Date:  2011-08-01       Impact factor: 5.191

6.  Linezolid-resistant Enterococcus faecalis isolated from a cord blood transplant recipient.

Authors:  Imad Dibo; Satish K Pillai; Howard S Gold; Maria R Baer; Meir Wetzler; James L Slack; Peggy A Hazamy; Donna Ball; Chiu Bin Hsiao; Philip L McCarthy; Brahm H Segal
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

7.  Mutations in the DNA mismatch repair proteins MutS and MutL of oxazolidinone-resistant or -susceptible Enterococcus faecium.

Authors:  Rob J Willems; Janetta Top; Derek J Smith; David I Roper; Sarah E North; Neil Woodford
Journal:  Antimicrob Agents Chemother       Date:  2003-10       Impact factor: 5.191

Review 8.  Resistance to macrolide, lincosamide, streptogramin, ketolide, and oxazolidinone antibiotics.

Authors:  Marilyn C Roberts
Journal:  Mol Biotechnol       Date:  2004-09       Impact factor: 2.695

9.  Emergence of clonal complex 17 Enterococcus faecium in The Netherlands.

Authors:  Janetta Top; Rob Willems; Saskia van der Velden; Miranda Asbroek; Marc Bonten
Journal:  J Clin Microbiol       Date:  2007-10-31       Impact factor: 5.948

10.  Molecular characterisation of linezolid resistance in two vancomycin-resistant (VanB) Enterococcus faecium isolates using Pyrosequencing.

Authors:  B Saager; H Rohde; B S Timmerbeil; G Franke; W Pothmann; J Dahlke; S Scherpe; I Sobottka; P Heisig; M A Horstkotte
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-04-18       Impact factor: 3.267
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