Literature DB >> 10523591

Novel method for rapid determination of clarithromycin sensitivity in Helicobacter pylori.

J R Gibson1, N A Saunders, B Burke, R J Owen.   

Abstract

A novel PCR-hybridization assay, performed in single closed capillaries, was developed to detect clarithromycin resistance-associated gene mutations in Helicobacter pylori. Mutations were detected by thermal analysis in 33 of 34 (97%) resistant isolates but not in 66 isolates determined to be sensitive by conventional antibiotic assays. The method was rapid and reproducible, and it reduced PCR product contamination risk.

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Year:  1999        PMID： 10523591      PMCID： PMC85748     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

Review 1.  Current European concepts in the management of Helicobacter pylori infection. The Maastricht Consensus Report. European Helicobacter Pylori Study Group.

Authors: 
Journal:  Gut       Date:  1997-07       Impact factor: 23.059

2.  The LightCycler: a microvolume multisample fluorimeter with rapid temperature control.

Authors:  C T Wittwer; K M Ririe; R V Andrew; D A David; R A Gundry; U J Balis
Journal:  Biotechniques       Date:  1997-01       Impact factor: 1.993

3.  A PCR-oligonucleotide ligation assay to determine the prevalence of 23S rRNA gene mutations in clarithromycin-resistant Helicobacter pylori.

Authors:  G G Stone; D Shortridge; J Versalovic; J Beyer; R K Flamm; D Y Graham; A T Ghoneim; S K Tanaka
Journal:  Antimicrob Agents Chemother       Date:  1997-03       Impact factor: 5.191

Review 4.  NIH Consensus Conference. Helicobacter pylori in peptic ulcer disease. NIH Consensus Development Panel on Helicobacter pylori in Peptic Ulcer Disease.

Authors: 
Journal:  JAMA       Date:  1994-07-06       Impact factor: 56.272

5.  Direct detection of Helicobacter pylori resistance to macrolides by a polymerase chain reaction/DNA enzyme immunoassay in gastric biopsy specimens.

Authors:  A Marais; L Monteiro; A Occhialini; M Pina; H Lamouliatte; F Mégraud
Journal:  Gut       Date:  1999-04       Impact factor: 23.059

6.  Macrolide resistance in Helicobacter pylori: rapid detection of point mutations and assays of macrolide binding to ribosomes.

Authors:  A Occhialini; M Urdaci; F Doucet-Populaire; C M Bébéar; H Lamouliatte; F Mégraud
Journal:  Antimicrob Agents Chemother       Date:  1997-12       Impact factor: 5.191

7.  Cloning and sequence analysis of two copies of a 23S rRNA gene from Helicobacter pylori and association of clarithromycin resistance with 23S rRNA mutations.

Authors:  D E Taylor; Z Ge; D Purych; T Lo; K Hiratsuka
Journal:  Antimicrob Agents Chemother       Date:  1997-12       Impact factor: 5.191

8.  Use of an amplified-fragment length polymorphism technique to fingerprint and differentiate isolates of Helicobacter pylori.

Authors:  J R Gibson; E Slater; J Xerry; D S Tompkins; R J Owen
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

9.  Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.

Authors:  J Versalovic; D Shortridge; K Kibler; M V Griffy; J Beyer; R K Flamm; S K Tanaka; D Y Graham; M F Go
Journal:  Antimicrob Agents Chemother       Date:  1996-02       Impact factor: 5.191

10.  Detection of point mutations associated with resistance of Helicobacter pylori to clarithromycin by hybridization in liquid phase.

Authors:  M Pina; A Occhialini; L Monteiro; H P Doermann; F Mégraud
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948
  10 in total
  23 in total

1.  Rapid real-time PCR for determination of penicillin susceptibility in pneumococcal meningitis, including culture-negative cases.

Authors:  Angela M Kearns; Clive Graham; Doreen Burdess; Jane Heatherington; Roger Freeman
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

2.  PCR-restriction fragment length polymorphism can also detect point mutation A2142C in the 23S rRNA gene, associated with Helicobacter pylori resistance to clarithromycin.

Authors:  Armelle Ménard; Adriana Santos; Francis Mégraud; Mónica Oleastro
Journal:  Antimicrob Agents Chemother       Date:  2002-04       Impact factor: 5.191

3.  LightCycler-based quantitative PCR for rapid detection of human herpesvirus 6 DNA in clinical material.

Authors:  A M Kearns; A J Turner; C E Taylor; P W George; R Freeman; A R Gennery
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

4.  Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis.

Authors:  Neil Woodford; Luke Tysall; Cressida Auckland; Mark W Stockdale; Andrew J Lawson; Rachel A Walker; David M Livermore
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

Review 5.  H pylori antibiotic resistance: prevalence, importance, and advances in testing.

Authors:  F Mégraud
Journal:  Gut       Date:  2004-09       Impact factor: 23.059

Review 6.  Clinical role and importance of fluorescence in situ hybridization method in diagnosis of H pylori infection and determination of clarithromycin resistance in H pylori eradication therapy.

Authors:  Ozlem Yilmaz; Ebru Demiray
Journal:  World J Gastroenterol       Date:  2007-02-07       Impact factor: 5.742

Review 7.  Antimicrobial susceptibility testing for Helicobacter pylori in times of increasing antibiotic resistance.

Authors:  Sinéad M Smith; Colm O'Morain; Deirdre McNamara
Journal:  World J Gastroenterol       Date:  2014-08-07       Impact factor: 5.742

8.  Identification of cagA tyrosine phosphorylation DNA motifs in Helicobacter pylori isolates from peptic ulcer patients by novel PCR-restriction fragment length polymorphism and real-time fluorescence PCR assays.

Authors:  Robert J Owen; Sally I Sharp; Stephanie A Chisholm; Sjoerd Rijpkema
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

Review 9.  Basis for the management of drug-resistant Helicobacter pylori infection.

Authors:  Francis Mégraud
Journal:  Drugs       Date:  2004       Impact factor: 9.546

10.  Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens.

Authors:  Claudia Schabereiter-Gurtner; Alexander M Hirschl; Brigitte Dragosics; Peter Hufnagl; Sonja Puz; Zsuzsanna Kovách; Manfred Rotter; Athanasios Makristathis
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948
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