PURPOSE: The aim of this study was to evaluate the effect of sperm single-stranded DNA, detected by acridine orange (AO), and classical sperm parameters on embryonic quality after ICSI. METHODS: Before ICSI, the spermatozoa of 183 infertile patients with oligo-, astheno-, teratozoospermia (n = 147), or more than one previous unsuccessful conventional IVF attempt (n = 36) were stained by AO to assess the presence of single-stranded DNA. Two days after ICSI, the embryos of 135 patients were scored for morphology, fragmentation included. Embryos of 48 couples were cultured for 4 days to develop to the morula or blastocyst stage. At most 2 embryos were transferred on Day 2 or 4. RESULTS: When the level of spermatozoa with single-stranded DNA was increased, there was a significantly lower fertilization rate after ICSI. Besides, increased sperm single-stranded DNA resulted in a higher proportion of heavily fragmented embryos on Day 2 (P < 0.05). In patients with an increased level of spermatozoa with single-stranded DNA, a significantly higher number of embryos were arrested in spite of prolonged culturing (P < 0.05). Classical sperm parameters did not affect the quality and developmental potential of ICSI-derived embryos. No correlation was found between the level of spermatozoa with single-stranded DNA, pregnancy rate, and live-birth rate achieved by ICSI, except in patients with 0% of spermatozoa with single-stranded DNA, in whom the pregnancy rate was significantly higher. CONCLUSIONS: Sperm single-stranded DNA provides additional data on sperm functional capacity in terms of fertilization and embryonic quality after ICSI.
PURPOSE: The aim of this study was to evaluate the effect of sperm single-stranded DNA, detected by acridine orange (AO), and classical sperm parameters on embryonic quality after ICSI. METHODS: Before ICSI, the spermatozoa of 183 infertilepatients with oligo-, astheno-, teratozoospermia (n = 147), or more than one previous unsuccessful conventional IVF attempt (n = 36) were stained by AO to assess the presence of single-stranded DNA. Two days after ICSI, the embryos of 135 patients were scored for morphology, fragmentation included. Embryos of 48 couples were cultured for 4 days to develop to the morula or blastocyst stage. At most 2 embryos were transferred on Day 2 or 4. RESULTS: When the level of spermatozoa with single-stranded DNA was increased, there was a significantly lower fertilization rate after ICSI. Besides, increased sperm single-stranded DNA resulted in a higher proportion of heavily fragmented embryos on Day 2 (P < 0.05). In patients with an increased level of spermatozoa with single-stranded DNA, a significantly higher number of embryos were arrested in spite of prolonged culturing (P < 0.05). Classical sperm parameters did not affect the quality and developmental potential of ICSI-derived embryos. No correlation was found between the level of spermatozoa with single-stranded DNA, pregnancy rate, and live-birth rate achieved by ICSI, except in patients with 0% of spermatozoa with single-stranded DNA, in whom the pregnancy rate was significantly higher. CONCLUSIONS: Sperm single-stranded DNA provides additional data on sperm functional capacity in terms of fertilization and embryonic quality after ICSI.
Authors: J Auger; F Eustache; B Ducot; T Blandin; M Daudin; I Diaz; S E Matribi; B Gony; L Keskes; M Kolbezen; A Lamarte; J Lornage; N Nomal; G Pitaval; O Simon; I Virant-Klun; A Spira; P Jouannet Journal: Hum Reprod Date: 2000-11 Impact factor: 6.918
Authors: Leandros A Lazaros; Georgios A Vartholomatos; Elissavet G Hatzi; Apostolos I Kaponis; Georgios V Makrydimas; Sophia N Kalantaridou; Nikolaos V Sofikitis; Theodoros Ioannis Stefos; Konstantinos A Zikopoulos; Ioannis A Georgiou Journal: J Assist Reprod Genet Date: 2011-07-21 Impact factor: 3.412
Authors: Giorgio Cavallini; Maria Cristina Magli; Andor Crippa; Anna Pia Ferraretti; Luca Gianaroli Journal: Asian J Androl Date: 2012-04-30 Impact factor: 3.285