Literature DB >> 6724015

A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence.

R I Tejada, J C Mitchell, A Norman, J J Marik, S Friedman.   

Abstract

A new and practical test for evaluating the fertility of a male subject has been developed. Twenty-eight donors whose semen had induced at least one pregnancy resulting in a normal delivery and 61 patients attending our infertility clinic were studied. Semen smears stained with acridine orange were read on a fluorescence microscope; sperm heads appeared either green (fertile) or red ( nonfertile ). We introduce the concept of an "effective sperm count" which is obtained by multiplying the percentage of green-fluorescing sperm by the actual sperm count. Of our fertile subjects, 27 of 28 (96.4%) exhibited an effective sperm count of greater than or equal to 50 million/ml, while 60 of 61 (98.3%) infertile patients fell below this value. The percent green correlates with neither actual sperm count nor motility, indicating that this test measures a new parameter of male fertility.

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Year:  1984        PMID: 6724015     DOI: 10.1016/s0015-0282(16)47963-x

Source DB:  PubMed          Journal:  Fertil Steril        ISSN: 0015-0282            Impact factor:   7.329


  69 in total

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Review 2.  Semen analysis in laboratory practice: an overview of routine tests.

Authors:  Fernando Tadeu Andrade-Rocha
Journal:  J Clin Lab Anal       Date:  2003       Impact factor: 2.352

3.  Relation between different human sperm nuclear maturity tests and in vitro fertilization.

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Journal:  J Assist Reprod Genet       Date:  2001-04       Impact factor: 3.412

4.  Association of various sperm parameters with unexplained repeated early pregnancy loss--which is most important?

Authors:  Sudhindra Mohan Bhattacharya
Journal:  Int Urol Nephrol       Date:  2008       Impact factor: 2.370

Review 5.  Male infertility: evaluation of human sperm function and its clinical application.

Authors:  A Lenzi
Journal:  J Endocrinol Invest       Date:  1995-06       Impact factor: 4.256

6.  Semen characteristics: Advancement in andrological assessment.

Authors:  R S Sharma; K K Gaur; P C Pal; Monika Manocha; Deepak Tomar; Arif Azam Khan; Vinita Tripathi; Vineeta Chattree; A Kriplani
Journal:  Indian J Clin Biochem       Date:  2005-01

7.  Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos.

Authors:  Irma Virant-Klun; Tomaz Tomazevic; Helena Meden-Vrtovec
Journal:  J Assist Reprod Genet       Date:  2002-07       Impact factor: 3.412

Review 8.  Sperm DNA damage in male infertility: etiologies, assays, and outcomes.

Authors:  Ryan T Schulte; Dana A Ohl; Mark Sigman; Gary D Smith
Journal:  J Assist Reprod Genet       Date:  2009-12-12       Impact factor: 3.412

9.  Relationship between sperm quality traits and field-fertility of porcine semen.

Authors:  I A Tsakmakidis; A G Lymberopoulos; T A Khalifa
Journal:  J Vet Sci       Date:  2010-06       Impact factor: 1.672

10.  The effect of varicocele on sperm morphology and DNA maturity: does acridine orange staining facilitate diagnosis?

Authors:  Ali Ersin Zümrütbaş; Ömer Gülpınar; Murat Mermerkaya; Evren Süer; Önder Yaman
Journal:  Turk J Urol       Date:  2013-09
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