| Literature DB >> 12167668 |
N H Dekker1, V V Rybenkov, M Duguet, N J Crisona, N R Cozzarelli, D Bensimon, V Croquette.
Abstract
The topology of cellular DNA is carefully controlled by enzymes called topoisomerases. By using single-molecule techniques, we monitored the activity of two type IA topoisomerases in real time under conditions in which single relaxation events were detected. The strict one-at-a-time removal of supercoils we observed establishes that these enzymes use an enzyme-bridged strand-passage mechanism that is well suited to their physiological roles and demonstrates a mechanistic unity with type II topoisomerases.Mesh：
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Year: 2002 PMID： 12167668 PMCID： PMC129409 DOI： 10.1073/pnas.132378799
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205