AIMS: The objective of the study was to optimize the submerged culture conditions for the production of exopolysaccharide from Paecilomyces sinclairii. METHODS AND RESULTS: The optimal temperature and initial pH for exopolysaccharide production by Paecilomyces sinclairii in shake flask culture were found to be 30 degrees C and 6.0, respectively. Sucrose (60 g l(-1)) and corn steep powder (10 g l(-1)) were the most suitable carbon and nitrogen source for exopolysaccharide production. CONCLUSIONS: Under optimal culture medium, the maximum exopolysaccharide concentration in a 5-l stirred-tank fermenter indicated 7.4 g l(-1), which was approximately three times higher than that in basal medium. The maximum specific growth rates (micro max) and yield coefficient (Y(P/S)) in the optimal culture medium was 0.16 h(-1) and 0.19, respectively. SIGNIFICANCE AND IMPACT OF THE STUDY: The optimal culture conditions reported in this article can be widely applied to the processes for submerged cultures of other mushrooms.
AIMS: The objective of the study was to optimize the submerged culture conditions for the production of exopolysaccharide from Paecilomyces sinclairii. METHODS AND RESULTS: The optimal temperature and initial pH for exopolysaccharide production by Paecilomyces sinclairii in shake flask culture were found to be 30 degrees C and 6.0, respectively. Sucrose (60 g l(-1)) and corn steep powder (10 g l(-1)) were the most suitable carbon and nitrogen source for exopolysaccharide production. CONCLUSIONS: Under optimal culture medium, the maximum exopolysaccharide concentration in a 5-l stirred-tank fermenter indicated 7.4 g l(-1), which was approximately three times higher than that in basal medium. The maximum specific growth rates (micro max) and yield coefficient (Y(P/S)) in the optimal culture medium was 0.16 h(-1) and 0.19, respectively. SIGNIFICANCE AND IMPACT OF THE STUDY: The optimal culture conditions reported in this article can be widely applied to the processes for submerged cultures of other mushrooms.
Authors: Sung Mi Shim; Yun Hee Oh; Kyung Rim Lee; Seong Hwan Kim; Kyung Hoan Im; Jung Wan Kim; U Youn Lee; Jae Ouk Shim; Mi Ja Shim; Min Woong Lee; Hyeon Su Ro; Hyun Sook Lee; Tae Soo Lee Journal: Mycobiology Date: 2005-03-31 Impact factor: 1.858
Authors: Sung Mi Shim; Yun Hee Oh; Kyung Rim Lee; Seong Hwan Kim; Kyung Hoan Im; Jung Wan Kim; U Youn Lee; Jae Ouk Shim; Mi Ja Shim; Min Woong Lee; Hyeon Su Ro; Hyun Sook Lee; Tae Soo Lee Journal: Mycobiology Date: 2005-03-31 Impact factor: 1.858