Literature DB >> 11885977

1H-filtered correlation experiments for assignment and determination of coupling constants in backbone labelled proteins.

Alexander W Giesen1, Lynnette C Bae, Christa L Barrett, Jason A Chyba, Michael M Chaykovsky, Minn-Chang Cheng, Jenny H Murray, Ed J Oliver, Sarah M Sullivan, Jonathan Miles Brown, Steve W Homans.   

Abstract

The implementation of [13Calpha,13C',15N,2Halpha] labelled amino acids into proteins allows the acquisition of high resolution triple resonance experiments. We present for the first time resonance assignments facilitated by this new labelling strategy. The absence of 1JCalpha,Cbeta couplings enables us to measure 1JCalpha,C' scalar and 1DCalpha,C' residual dipolar coupling constants using modified HNCA experiments which do not suffer from sensitivity losses characteristic for 13C constant time experiments.

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Year:  2002        PMID： 11885977     DOI： 10.1023/a:1013831417615

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  11 in total

1.  A J(CH)-modulated 2D (HACACO)NH pulse scheme for quantitative measurement of (13)C(alpha)-(1)H(alpha) couplings in (15)N, (13)C-labeled proteins.

Authors:  T K Hitchens; S A McCallum; G S Rule
Journal:  J Magn Reson       Date:  1999-09       Impact factor: 2.229

2.  A new approach for obtaining sequential assignment of large proteins.

Authors:  P Permi; A Annila
Journal:  J Biomol NMR       Date:  2001-06       Impact factor: 2.835

3.  Increased sensitivity in HNCA and HN(CO)CA experiments by selective C beta decoupling.

Authors:  H Matsuo; E Kupce; H Li; G Wagner
Journal:  J Magn Reson B       Date:  1996-10

Review 4.  Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra.

Authors:  M Ottiger; F Delaglio; A Bax
Journal:  J Magn Reson       Date:  1998-04       Impact factor: 2.229

5.  Determination of H(N),H (α) and H (N),C' coupling constants in (13)C, (15)N-labeled proteins.

Authors:  R Weisemann; H Rüterjans; H Schwalbe; J Schleucher; W Bermel; C Griesinger
Journal:  J Biomol NMR       Date:  1994-03       Impact factor: 2.835

6.  A quantitative J-correlation experiment for the accurate measurement of one-bond amide 15N-1H couplings in proteins.

Authors:  J R Tolman; J H Prestegard
Journal:  J Magn Reson B       Date:  1996-09

7.  A set of HNCO-based experiments for measurement of residual dipolar couplings in 15N, 13C, (2H)-labeled proteins.

Authors:  P Permi; P R Rosevear; A Annila
Journal:  J Biomol NMR       Date:  2000-05       Impact factor: 2.835

8.  [13C]-constant-time [15N,1H]-TROSY-HNCA for sequential assignments of large proteins.

Authors:  M Salzmann; K Pervushin; G Wider; H Senn; K Wüthrich
Journal:  J Biomol NMR       Date:  1999-05       Impact factor: 2.835

9.  Characterizing the use of perdeuteration in NMR studies of large proteins: 13C, 15N and 1H assignments of human carbonic anhydrase II.

Authors:  R A Venters; B T Farmer; C A Fierke; L D Spicer
Journal:  J Mol Biol       Date:  1996-12-20       Impact factor: 5.469

10.  13C-NOESY-HSQC with Split Carbon Evolution for Increased Resolution with Uniformly Labeled Proteins.

Authors: 
Journal:  J Magn Reson       Date:  1998-06       Impact factor: 2.229
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  1 in total

1.  A set of HA-detected experiments for measuring scalar and residual dipolar couplings.

Authors:  Peter Würtz; Kai Fredriksson; Perttu Permi
Journal:  J Biomol NMR       Date:  2005-04       Impact factor: 2.835
  1 in total

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