Literature DB >> 11727979

Improved TROSY-HNCA experiment with suppression of conformational exchange induced relaxation.

K Pervushin1, V Gallius, C Ritter.   

Abstract

A general method for improving of the sensitivity of the TROSY-type triple resonance experiments in the presence of conformational exchange-induced (CSX) relaxation is proposed based on the use of CPMG-INEPT (Müller et al., J. Am. Chem. Soc., 1995, 117, 11043-11048) during the N-C polarization transfer periods. Significantly improved sensitivity is demonstrated for the majority of cross-peaks in the new [15N,1H]-TROSY-XY-HNCA experiment, measured with partially folded RNase AS-Protein, with negligible loss of sensitivity for resonances unaffected by CSX relaxation. In addition, a comparison of cross-peak amplitudes in [15N,1N]-TROSY-XY-HNCA and conventional [15N,1H]-TROSY-HNCA spectra provides a quick and sensitive estimation of the CSX relaxation contribution.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11727979     DOI： 10.1023/a:1012484027195

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  14 in total

1.  The 3D NOESY-[(1)H,(15)N,(1)H]-ZQ-TROSY NMR experiment with diagonal peak suppression.

Authors:  K V Pervushin; G Wider; R Riek; K Wüthrich
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-17       Impact factor: 11.205

2.  Improved HSQC experiments for the observation of exchange broadened signals.

Authors:  F A Mulder; C A Spronk; M Slijper; R Kaptein; R Boelens
Journal:  J Biomol NMR       Date:  1996-09       Impact factor: 2.835

3.  Recognition of local glycoprotein misfolding by the ER folding sensor UDP-glucose:glycoprotein glucosyltransferase.

Authors:  C Ritter; A Helenius
Journal:  Nat Struct Biol       Date:  2000-04

4.  Comprehensive NOE characterization of a partially folded large fragment of staphylococcal nuclease Delta131Delta, using NMR methods with improved resolution.

Authors:  O Zhang; L E Kay; D Shortle; J D Forman-Kay
Journal:  J Mol Biol       Date:  1997-09-12       Impact factor: 5.469

5.  Improved sensitivity of HSQC spectra of exchanging protons at short interscan delays using a new fast HSQC (FHSQC) detection scheme that avoids water saturation.

Authors:  S Mori; C Abeygunawardana; M O Johnson; P C van Zijl
Journal:  J Magn Reson B       Date:  1995-07

6.  TROSY NMR with partially deuterated proteins.

Authors:  A Eletsky; A Kienhöfer; K Pervushin
Journal:  J Biomol NMR       Date:  2001-06       Impact factor: 2.835

7.  Triple-resonance NOESY-based experiments with improved spectral resolution: applications to structural characterization of unfolded, partially folded and folded proteins.

Authors:  O Zhang; J D Forman-Kay; D Shortle; L E Kay
Journal:  J Biomol NMR       Date:  1997-02       Impact factor: 2.835

8.  Protein dynamics studied by rotating frame 15N spin relaxation times.

Authors:  T Szyperski; P Luginbühl; G Otting; P Güntert; K Wüthrich
Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835

9.  Refinement of the crystal structure of ribonuclease S. Comparison with and between the various ribonuclease A structures.

Authors:  E E Kim; R Varadarajan; H W Wyckoff; F M Richards
Journal:  Biochemistry       Date:  1992-12-15       Impact factor: 3.162

10.  Conformational requirements for glycoprotein reglucosylation in the endoplasmic reticulum.

Authors:  E S Trombetta; A Helenius
Journal:  J Cell Biol       Date:  2000-03-20       Impact factor: 10.539
View more
  3 in total

1.  The receptor-CheW binding interface in bacterial chemotaxis.

Authors:  Anh Vu; Xiqing Wang; Hongjun Zhou; Frederick W Dahlquist
Journal:  J Mol Biol       Date:  2011-12-06       Impact factor: 5.469

2.  Introducing 77Se NMR Spectroscopy to Analyzing Galectin -Ligand Interaction.

Authors:  Mária Raics; István Timári; László Szilágyi; Hans-Joachim Gabius; Katalin E Kövér
Journal:  Methods Mol Biol       Date:  2022

3.  Selenoglycosides as Lectin Ligands: 77 Se-Edited CPMG-HSQMBC NMR Spectroscopy To Monitor Biomedically Relevant Interactions.

Authors:  Mária Raics; István Timári; Tammo Diercks; László Szilágyi; Hans-Joachim Gabius; Katalin E Kövér
Journal:  Chembiochem       Date:  2019-06-05       Impact factor: 3.164
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.