Literature DB >> 11682549

Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens.

E Collins1, M Glennon, S Hanley, A M Murray, M Cormican, T Smith, M Maher.   

Abstract

DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.

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Year:  2001        PMID: 11682549      PMCID: PMC88506          DOI: 10.1128/JCM.39.11.4163-4165.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  11 in total

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Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

2.  PCR for the detection of Campylobacter spp. in clinical specimens.

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3.  PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples.

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4.  Rapid and effective method for preparation of fecal specimens for PCR assays.

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5.  Detection of Helicobacter pylori DNA in fecal samples from infected individuals.

Authors:  W A Gramley; A Asghar; H F Frierson; S M Powell
Journal:  J Clin Microbiol       Date:  1999-07       Impact factor: 5.948

6.  PCR-ELISAs for the detection of Campylobacter jejuni and Campylobacter coli in poultry samples.

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7.  Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay.

Authors:  A J Lawson; J M Logan; G L O'neill; M Desai; J Stanley
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8.  Detection and differentiation of Campylobacter jejuni and Campylobacter coli in broiler chicken samples using a PCR/DNA probe membrane based colorimetric detection assay.

Authors:  N A O'Sullivan; R Fallon; C Carroll; T Smith; M Maher
Journal:  Mol Cell Probes       Date:  2000-02       Impact factor: 2.365

9.  PCR detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA.

Authors:  J L Holland; L Louie; A E Simor; M Louie
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

10.  PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: application to isolates and stool samples.

Authors:  L A Metherell; J M Logan; J Stanley
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

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  5 in total

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Authors:  Majella Maher; Cathriona Finnegan; Evelyn Collins; Brid Ward; Cyril Carroll; Martin Cormican
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2.  Use of PCR for direct detection of Campylobacter species in bovine feces.

Authors:  G Douglas Inglis; Lisa D Kalischuk
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

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Authors:  David R Tribble; Shahida Baqar; Lorrin W Pang; Carl Mason; Huo-Shu H Houng; Chittima Pitarangsi; Carlos Lebron; Adam Armstrong; Orntipa Sethabutr; John W Sanders
Journal:  J Clin Microbiol       Date:  2008-01-30       Impact factor: 5.948

4.  Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools.

Authors:  Hiroshi Fukushima; Yoshie Tsunomori; Ryotaro Seki
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5.  Development and evaluation of immunochromatographic assay for simple and rapid detection of Campylobacter jejuni and Campylobacter coli in human stool specimens.

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  5 in total

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