Literature DB >> 9889235

PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: application to isolates and stool samples.

L A Metherell1, J M Logan, J Stanley.   

Abstract

We report a PCR-enzyme-linked immunosorbent assay which identifies Campylobacter species by capture hybridization of a single-stranded 16S rRNA gene amplicon with species-specific probes in a microtiter plate format. Specificities were confirmed for both reference and field strains, but the type strain of Campylobacter coli was atypical. The assay was rapid, informative, and usable with stool-extracted DNA.

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Year:  1999        PMID： 9889235      PMCID： PMC84331     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  PCR-enzyme-linked immunosorbent assay and partial rRNA gene sequencing: a rational approach to identifying mycobacteria.

Authors:  S Patel; M Yates; N A Saunders
Journal:  J Clin Microbiol       Date:  1997-09       Impact factor: 5.948

2.  CLUSTAL V: improved software for multiple sequence alignment.

Authors:  D G Higgins; A J Bleasby; R Fuchs
Journal:  Comput Appl Biosci       Date:  1992-04

3.  PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples.

Authors:  D Linton; A J Lawson; R J Owen; J Stanley
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

4.  Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals.

Authors:  D Linton; R J Owen; J Stanley
Journal:  Res Microbiol       Date:  1996 Nov-Dec       Impact factor: 3.992

5.  Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa.

Authors:  R A Clayton; G Sutton; P S Hinkle; C Bult; C Fields
Journal:  Int J Syst Bacteriol       Date:  1995-07

6.  Polymerase chain reaction detection and speciation of Campylobacter upsaliensis and C. helveticus in human faeces and comparison with culture techniques.

Authors:  A J Lawson; D Linton; J Stanley; R J Owen
Journal:  J Appl Microbiol       Date:  1997-09       Impact factor: 3.772

7.  Chemotaxonomic analyses of Bacteroides gracilis and Bacteroides ureolyticus and reclassification of B. gracilis as Campylobacter gracilis comb. nov.

Authors:  P Vandamme; M I Daneshvar; F E Dewhirst; B J Paster; K Kersters; H Goossens; C W Moss
Journal:  Int J Syst Bacteriol       Date:  1995-01
  7 in total
  14 in total

1.  Evaluation of the Alexon-trend ProSpecT Campylobacter microplate assay.

Authors:  R Tolcin; M M LaSalvia; B A Kirkley; E A Vetter; F R Cockerill; G W Procop
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

Review 2.  Specificity and performance of PCR detection assays for microbial pathogens.

Authors:  Konrad Sachse
Journal:  Mol Biotechnol       Date:  2004-01       Impact factor: 2.695

3.  Detection of Campylobacter spp. in chicken fecal samples by real-time PCR.

Authors:  Marianne Lund; Steen Nordentoft; Karl Pedersen; Mogens Madsen
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

4.  Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens.

Authors:  E Collins; M Glennon; S Hanley; A M Murray; M Cormican; T Smith; M Maher
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

5.  Rapid identification of Campylobacter spp. by melting peak analysis of biprobes in real-time PCR.

Authors:  J M Logan; K J Edwards; N A Saunders; J Stanley
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

6.  Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay.

Authors:  A J Lawson; J M Logan; G L O'neill; M Desai; J Stanley
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

7.  Basis of the superiority of cefoperazone amphotericin teicoplanin for isolating Campylobacter upsaliensis from stools.

Authors:  C Byrne; D Doherty; A Mooney; M Byrne; D Woodward; W Johnson; F Rodgers; B Bourke
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

8.  Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA.

Authors:  John D Klena; Craig T Parker; Krista Knibb; J Claire Ibbitt; Phillippa M L Devane; Sharon T Horn; William G Miller; Michael E Konkel
Journal:  J Clin Microbiol       Date:  2004-12       Impact factor: 5.948

9.  Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces.

Authors:  Majella Maher; Cathriona Finnegan; Evelyn Collins; Brid Ward; Cyril Carroll; Martin Cormican
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

10.  Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods.

Authors:  S P Kulkarni; S Lever; J M J Logan; A J Lawson; J Stanley; M S Shafi
Journal:  J Clin Pathol       Date:  2002-10       Impact factor: 3.411
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