Literature DB >> 10364591

Detection of Helicobacter pylori DNA in fecal samples from infected individuals.

W A Gramley1, A Asghar, H F Frierson, S M Powell.   

Abstract

Stool, gastric biopsy, and serum samples were collected from 22 subjects. DNA from stool was extracted, amplified, and hybridized with primers specific for the 16S rRNA gene of Helicobacter pylori. DNA from gastric biopsy specimens was analyzed similarly for comparison. Universal primers were used to confirm successful extraction of DNA from samples. Histologic, serologic, and DNA analyses were scored in a blinded fashion. Universal primer amplification verified successful DNA extraction from all stool and gastric tissue specimens. The gastric tissue DNA assay was positive for H. pylori in 11 of the 22 subjects, correlating completely with histologic and serologic results. Stool DNA was positive for H. pylori by our molecular assay in 8 of these 11 H. pylori-positive subjects. All subjects who were negative by histologic, serologic, and gastric tissue DNA analyses were also negative by stool DNA analysis. Compared to histology, serology, and gastric tissue DNA analyses, the sensitivity of our stool DNA assay was 73%, with a specificity of 100%.

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Year:  1999        PMID： 10364591      PMCID： PMC85126     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  24 in total

1.  Detection and serotyping of rotaviruses in stool specimens by using reverse transcription and polymerase chain reaction amplification.

Authors:  H Ushijima; H Koike; A Mukoyama; A Hasegawa; S Nishimura; J Gentsch
Journal:  J Med Virol       Date:  1992-12       Impact factor: 2.327

2.  Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.

Authors:  S A Ho; J A Hoyle; F A Lewis; A D Secker; D Cross; N P Mapstone; M F Dixon; J I Wyatt; D S Tompkins; G R Taylor
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

3.  Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture-multiplex PCR combination assay.

Authors:  C H Chiu; J T Ou
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

4.  PCR identification of Helicobacter pylori in faeces from gastritis patients.

Authors:  N P Mapstone; D A Lynch; F A Lewis; A T Axon; D S Tompkins; M F Dixon; P Quirke
Journal:  Lancet       Date:  1993-02-13       Impact factor: 79.321

5.  Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors.

Authors:  D Sidransky; T Tokino; S R Hamilton; K W Kinzler; B Levin; P Frost; B Vogelstein
Journal:  Science       Date:  1992-04-03       Impact factor: 47.728

6.  Isolation of Helicobacter pylori from human faeces.

Authors:  J E Thomas; G R Gibson; M K Darboe; A Dale; L T Weaver
Journal:  Lancet       Date:  1992-11-14       Impact factor: 79.321

7.  Comparison of PCR and other diagnostic techniques for detection of Helicobacter pylori infection in dyspeptic patients.

Authors:  J Weiss; J Mecca; E da Silva; D Gassner
Journal:  J Clin Microbiol       Date:  1994-07       Impact factor: 5.948

8.  Helicobacter pylori infection and the risk of gastric carcinoma.

Authors:  J Parsonnet; G D Friedman; D P Vandersteen; Y Chang; J H Vogelman; N Orentreich; R K Sibley
Journal:  N Engl J Med       Date:  1991-10-17       Impact factor: 91.245

9.  Sensitive detection of Helicobacter pylori by using polymerase chain reaction.

Authors:  C L Clayton; H Kleanthous; P J Coates; D D Morgan; S Tabaqchali
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

10.  Use of PCR with feces for detection of Helicobacter pylori infections in patients.

Authors:  A A van Zwet; J C Thijs; A M Kooistra-Smid; J Schirm; J A Snijder
Journal:  J Clin Microbiol       Date:  1994-05       Impact factor: 5.948
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  22 in total

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Authors:  N Fernando; J Holton; D Vaira; M DeSilva; D Fernando
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

Review 2.  Clinical role and importance of fluorescence in situ hybridization method in diagnosis of H pylori infection and determination of clarithromycin resistance in H pylori eradication therapy.

Authors:  Ozlem Yilmaz; Ebru Demiray
Journal:  World J Gastroenterol       Date:  2007-02-07       Impact factor: 5.742

3.  Pretreatment with urea-hydrochloric acid enhances the isolation of Helicobacter pylori from contaminated specimens.

Authors:  Q Song; G W Zirnstein; B Swaminathan; B D Gold
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

4.  Five methods for detection of Helicobacter pylori in the Turkish population.

Authors:  Orhan Cem Aktepe; Ihsan Hakkı Ciftçi; Birol Safak; Ihsan Uslan; Fatma Hüsniye Dilek
Journal:  World J Gastroenterol       Date:  2011-12-21       Impact factor: 5.742

5.  Ticks and tick-borne pathogens in livestock from nomadic herds in the Somali Region, Ethiopia.

Authors:  Laura Tomassone; E Grego; G Callà; P Rodighiero; G Pressi; S Gebre; B Zeleke; D De Meneghi
Journal:  Exp Appl Acarol       Date:  2012-02-17       Impact factor: 2.132

6.  Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens.

Authors:  E Collins; M Glennon; S Hanley; A M Murray; M Cormican; T Smith; M Maher
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

7.  Are Helicobacter species and enterotoxigenic Bacteroides fragilis involved in inflammatory bowel disease?

Authors:  Christelle Basset; John Holton; Alexandra Bazeos; Dino Vaira; Stuart Bloom
Journal:  Dig Dis Sci       Date:  2004-09       Impact factor: 3.199

8.  Comparison of diagnostic techniques for Helicobacter cetorum infection in wild Atlantic bottlenose dolphins (Tursiops truncatus).

Authors:  Claudia G Harper; Mark T Whary; Yan Feng; Howard L Rhinehart; Randall S Wells; Shilu Xu; Nancy S Taylor; James G Fox
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

9.  Species of the family Helicobacteraceae detected in an Australian sea lion (Neophoca cinerea) with chronic gastritis.

Authors:  Andrew P A Oxley; Mark Powell; David B McKay
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948

10.  Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens.

Authors:  Claudia Schabereiter-Gurtner; Alexander M Hirschl; Brigitte Dragosics; Peter Hufnagl; Sonja Puz; Zsuzsanna Kovách; Manfred Rotter; Athanasios Makristathis
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948
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