Literature DB >> 11593008

Visualization of protein S1 within the 30S ribosomal subunit and its interaction with messenger RNA.

J Sengupta1, R K Agrawal, J Frank.   

Abstract

S1 is the largest ribosomal protein, present in the small subunit of the bacterial ribosome. It has a pivotal role in stabilizing the mRNA on the ribosome. Thus far, S1 has eluded structural determination. We have identified the S1 protein mass in the cryo-electron microscopic map of the Escherichia coli ribosome by comparing the map with a recent x-ray crystallographic structure of the 30S subunit, which lacks S1. According to our finding, S1 is located at the junction of head, platform, and main body of the 30S subunit, thus explaining all existing biochemical and crosslinking data. Protein S1 as identified in our map has a complex, elongated shape with two holes in its central portion. The N-terminal domain, forming one of the extensions, penetrates into the head of the 30S subunit. Evidence for direct interaction of S1 with 11 nucleotides of the mRNA, immediately upstream of the Shine-Dalgarno sequence, explains the protein's role in the recognition of the 5' region of mRNA.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11593008      PMCID： PMC59823          DOI： 10.1073/pnas.211266898

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  49 in total

1.  The small ribosomal subunit from Thermus thermophilus at 4.5 A resolution: pattern fittings and the identification of a functional site.

Authors:  A Tocilj; F Schlünzen; D Janell; M Glühmann; H A Hansen; J Harms; A Bashan; H Bartels; I Agmon; F Franceschi; A Yonath
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-07       Impact factor: 11.205

2.  Ribosomal components required for binding protein S1 to the 30 S subunit of Escherichia coli.

Authors:  M Laughrea; P B Moore
Journal:  J Mol Biol       Date:  1978-06-15       Impact factor: 5.469

3.  Functional domains of Escherichia coli Ribosomal Protein S1. Formation and characterization of a fragment with ribosome-binding properties.

Authors:  T Suryanarayana; A R Subramanian
Journal:  J Mol Biol       Date:  1979-01-05       Impact factor: 5.469

4.  Exchange of individual ribosomal proteins between ribosomes as studied by heavy isotope-transfer experiments.

Authors:  A R Subramanian; J van Duin
Journal:  Mol Gen Genet       Date:  1977-12-14

5.  Differential requirements for polypeptide chain initiation complex formation at the three bacteriophage R17 initiator regions.

Authors:  J A Steitz; A J Wahba; M Laughrea; P B Moore
Journal:  Nucleic Acids Res       Date:  1977-01       Impact factor: 16.971

6.  Positions of S2, S13, S16, S17, S19 and S21 in the 30 S ribosomal subunit of Escherichia coli.

Authors:  M S Capel; M Kjeldgaard; D M Engelman; P B Moore
Journal:  J Mol Biol       Date:  1988-03-05       Impact factor: 5.469

7.  An essential function of ribosomal protein S1 in messenger ribonucleic acid translation.

Authors:  T Suryanarayana; A R Subramanian
Journal:  Biochemistry       Date:  1983-05-24       Impact factor: 3.162

8.  Positions of proteins S6, S11 and S15 in the 30 S ribosomal subunit of Escherichia coli.

Authors:  V R Ramakrishnan; S Yabuki; I Y Sillers; D G Schindler; D M Engelman; P B Moore
Journal:  J Mol Biol       Date:  1981-12-15       Impact factor: 5.469

9.  RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment with bis-(2-chloroethyl)-methylamine.

Authors:  B Greuer; M Osswald; R Brimacombe; G Stöffler
Journal:  Nucleic Acids Res       Date:  1987-04-24       Impact factor: 16.971

10.  Function of the repeating homologous sequences in nucleic acid binding domain of ribosomal protein S1.

Authors:  T Suryanarayana; A R Subramanian
Journal:  Biochemistry       Date:  1984-03-13       Impact factor: 3.162
View more
  68 in total

1.  Protein S1 counteracts the inhibitory effect of the extended Shine-Dalgarno sequence on translation.

Authors:  Anastassia V Komarova; Ludmila S Tchufistova; Elena V Supina; Irina V Boni
Journal:  RNA       Date:  2002-09       Impact factor: 4.942

2.  Comparative analysis of ribosomal proteins in complete genomes: an example of reductive evolution at the domain scale.

Authors:  Odile Lecompte; Raymond Ripp; Jean-Claude Thierry; Dino Moras; Olivier Poch
Journal:  Nucleic Acids Res       Date:  2002-12-15       Impact factor: 16.971

3.  Correlations between Shine-Dalgarno sequences and gene features such as predicted expression levels and operon structures.

Authors:  Jiong Ma; Allan Campbell; Samuel Karlin
Journal:  J Bacteriol       Date:  2002-10       Impact factor: 3.490

4.  Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules.

Authors:  Kathleen E McGinness; Robert T Sauer
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-31       Impact factor: 11.205

5.  Robust translation of the nucleoid protein Fis requires a remote upstream AU element and is enhanced by RNA secondary structure.

Authors:  Maryam Nafissi; Jeannette Chau; Jimin Xu; Reid C Johnson
Journal:  J Bacteriol       Date:  2012-03-02       Impact factor: 3.490

6.  Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor.

Authors:  Ahmad Jomaa; Geordie Stewart; Jason A Mears; Inga Kireeva; Eric D Brown; Joaquin Ortega
Journal:  RNA       Date:  2011-09-29       Impact factor: 4.942

Review 7.  One core, two shells: bacterial and eukaryotic ribosomes.

Authors:  Sergey Melnikov; Adam Ben-Shem; Nicolas Garreau de Loubresse; Lasse Jenner; Gulnara Yusupova; Marat Yusupov
Journal:  Nat Struct Mol Biol       Date:  2012-06-05       Impact factor: 15.369

Review 8.  Initiation of protein synthesis in bacteria.

Authors:  Brian Søgaard Laursen; Hans Peter Sørensen; Kim Kusk Mortensen; Hans Uffe Sperling-Petersen
Journal:  Microbiol Mol Biol Rev       Date:  2005-03       Impact factor: 11.056

9.  Genomic and proteomic comparisons between bacterial and archaeal genomes and related comparisons with the yeast and fly genomes.

Authors:  Samuel Karlin; Luciano Brocchieri; Allan Campbell; Martha Cyert; Jan Mrázek
Journal:  Proc Natl Acad Sci U S A       Date:  2005-05-09       Impact factor: 11.205

10.  Ribosomes are optimized for autocatalytic production.

Authors:  Shlomi Reuveni; Måns Ehrenberg; Johan Paulsson
Journal:  Nature       Date:  2017-07-19       Impact factor: 49.962
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.