Literature DB >> 11574614

Simple and rational approach to the identification of Mycobacterium tuberculosis, Mycobacterium avium complex species, and other commonly isolated mycobacteria.

D A Wong1, P C Yip, D T Cheung, K M Kam.   

Abstract

A novel PCR-restriction fragment length polymorphism analysis of the hsp65 gene was developed. The restriction patterns for Mycobacterium tuberculosis and Mycobacterium avium complex (MAC) species were designed to be highly distinct, and the overall number of restriction patterns was designed to be limited. Four hundred specimens (17 reference strains and 383 clinical isolates) were tested, of which 98 were M. tuberculosis and 132 were MAC species. The assay was virtually 100% sensitive and specific for M. tuberculosis and MAC species. Moreover, it gave highly concordant results for other mycobacterial species other than M. terrae complex species. This assay can be completed in one day and is user-friendly and robust. Therefore, it is highly suitable for large-scale use in a clinical laboratory.

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Year:  2001        PMID： 11574614      PMCID： PMC88430          DOI： 10.1128/JCM.39.10.3768-3771.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Sequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA bacterial identification system.

Authors:  J B Patel; D G Leonard; X Pan; J M Musser; R E Berman; I Nachamkin
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

2.  Species identification of mycobacteria by PCR-restriction fragment length polymorphism of the rpoB gene.

Authors:  H Lee; H J Park; S N Cho; G H Bai; S J Kim
Journal:  J Clin Microbiol       Date:  2000-08       Impact factor: 5.948

3.  Differentiation of Mycobacterium species by direct sequencing of amplified DNA.

Authors:  T Rogall; T Flohr; E C Böttger
Journal:  J Gen Microbiol       Date:  1990-09

4.  Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.

Authors:  P Kirschner; B Springer; U Vogel; A Meier; A Wrede; M Kiekenbeck; F C Bange; E C Böttger
Journal:  J Clin Microbiol       Date:  1993-11       Impact factor: 5.948

5.  Rapid identification of mycobacteria to species level by PCR-restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species.

Authors:  A Devallois; K S Goh; N Rastogi
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

6.  Separation among species of Mycobacterium terrae complex by lipid analyses: comparison with biochemical tests and 16S rRNA sequencing.

Authors:  P Torkko; M Suutari; S Suomalainen; L Paulin; L Larsson; M L Katila
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

7.  Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.

Authors:  A Telenti; F Marchesi; M Balz; F Bally; E C Böttger; T Bodmer
Journal:  J Clin Microbiol       Date:  1993-02       Impact factor: 5.948

8.  Identification of Mycobacterium kansasii by using a DNA probe (AccuProbe) and molecular techniques.

Authors:  E Richter; S Niemann; S Rüsch-Gerdes; S Hoffner
Journal:  J Clin Microbiol       Date:  1999-04       Impact factor: 5.948

9.  Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S-23S rRNA gene spacer and restriction endonucleases.

Authors:  A Roth; U Reischl; A Streubel; L Naumann; R M Kroppenstedt; M Habicht; M Fischer; H Mauch
Journal:  J Clin Microbiol       Date:  2000-03       Impact factor: 5.948

10.  IS6110 dot blot hybridization for the identification of Mycobacterium tuberculosis complex.

Authors:  K M Kam; C W Yip; M Y Chan; C Y Mok; W S Wong
Journal:  Diagn Microbiol Infect Dis       Date:  1999-01       Impact factor: 2.803
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  16 in total

1.  Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes.

Authors:  Jean-Noël Dauendorffer; Isabelle Guillemin; Alexandra Aubry; Chantal Truffot-Pernot; Wladimir Sougakoff; Vincent Jarlier; Emmanuelle Cambau
Journal:  J Clin Microbiol       Date:  2003-03       Impact factor: 5.948

2.  Web-accessible database of hsp65 sequences from Mycobacterium reference strains.

Authors:  Jianli Dai; Yuansha Chen; Michael Lauzardo
Journal:  J Clin Microbiol       Date:  2011-03-30       Impact factor: 5.948

3.  Rapid method for identification of six common species of mycobacteria based on multiplex SNP analysis.

Authors:  Hongxiu Wang; Jun Yue; Min Han; Jinghui Yang; Yanlin Zhao
Journal:  J Clin Microbiol       Date:  2009-11-04       Impact factor: 5.948

4.  Guidelines for the prevention and treatment of opportunistic infections in HIV-exposed and HIV-infected children: recommendations from the National Institutes of Health, Centers for Disease Control and Prevention, the HIV Medicine Association of the Infectious Diseases Society of America, the Pediatric Infectious Diseases Society, and the American Academy of Pediatrics.

Authors:  George K Siberry; Mark J Abzug; Sharon Nachman; Michael T Brady; Kenneth L Dominguez; Edward Handelsman; Lynne M Mofenson; Steve Nesheim
Journal:  Pediatr Infect Dis J       Date:  2013-11       Impact factor: 2.129

5.  Rapid and simultaneous detection of Mycobacterium tuberculosis complex and Beijing/W genotype in sputum by an optimized DNA extraction protocol and a novel multiplex real-time PCR.

Authors:  Eric T Y Leung; L Zheng; Rity Y K Wong; Edward W C Chan; T K Au; Raphael C Y Chan; Grace Lui; Nelson Lee; Margaret Ip
Journal:  J Clin Microbiol       Date:  2011-05-18       Impact factor: 5.948

6.  Atypical mycobacterial exit-site infection and peritonitis in peritoneal dialysis patients on prophylactic exit-site gentamicin cream.

Authors:  Man-wai Lo; Siu-ka Mak; Yuk-yi Wong; Kwok-chi Lo; Shuk-fan Chan; Gensy M W Tong; Kin-yee Lo; Ping-nam Wong; Cindy W S Tse; Kai-Man Kam; Andrew K M Wong
Journal:  Perit Dial Int       Date:  2012-10-02       Impact factor: 1.756

7.  Commercial Disinfectants During Disinfection Process Validation: More Failures than Success.

Authors:  Shiv Sekhar Chatterjee; Sushil Kumar Chumber; Uma Khanduri
Journal:  J Clin Diagn Res       Date:  2016-08-01

8.  Guidelines for the Prevention and Treatment of Opportunistic Infections among HIV-exposed and HIV-infected children: recommendations from CDC, the National Institutes of Health, the HIV Medicine Association of the Infectious Diseases Society of America, the Pediatric Infectious Diseases Society, and the American Academy of Pediatrics.

Authors:  Lynne M Mofenson; Michael T Brady; Susie P Danner; Kenneth L Dominguez; Rohan Hazra; Edward Handelsman; Peter Havens; Steve Nesheim; Jennifer S Read; Leslie Serchuck; Russell Van Dyke
Journal:  MMWR Recomm Rep       Date:  2009-09-04

9.  Comparison of intracellular cytokine flow cytometry and an enzyme immunoassay for evaluation of cellular immune response to active tuberculosis.

Authors:  Wai Lin Leung; Kai Leung Law; Veronica Sui Shan Leung; Chi Wai Yip; Chi Chiu Leung; Cheuk Ming Tam; Kai Man Kam
Journal:  Clin Vaccine Immunol       Date:  2009-01-07

10.  Development of a novel PCR restriction analysis of the hsp65 gene as a rapid method to screen for the Mycobacterium tuberculosis complex and nontuberculous mycobacteria in high-burden countries.

Authors:  Mandira Varma-Basil; Kushal Garima; Rakesh Pathak; Shailendra Kumar Dhar Dwivedi; Anshika Narang; Anuj Bhatnagar; Mridula Bose
Journal:  J Clin Microbiol       Date:  2013-01-30       Impact factor: 5.948
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