Shiv Sekhar Chatterjee1, Sushil Kumar Chumber2, Uma Khanduri3. 1. Assistant Professor, Department of Microbiology, Nil Ratan Sircar Medical College , Kolkata, India . 2. Senior Consultant and In-charge, Department of Microbiology and Infection Control, St Stephen Hospital , Tis Hazari, Delhi, India . 3. Senior Consultant, Hematology and Head of the Department, Department of Laboratory Medicine, St Stephen Hospital , Tis Hazari, Delhi, India .
Abstract
INTRODUCTION: Disinfection process validation is mandatory before introduction of a new disinfectant in hospital services. Commercial disinfection brands often question existing hospital policy claiming greater efficacy and lack of toxicity of their products. Inadvertent inadequate disinfection leads to morbidity, patient's economic burden, and the risk of mortality. AIM: To evaluate commercial disinfectants for high, intermediate and low-level disinfection so as to identify utility for our routine situations. MATERIALS AND METHODS: This laboratory based experiment was conducted at St Stephen Hospital, Delhi during July-September 2013. Twelve commercial disinfectants: Sanidex®, Sanocid®, Cidex®, SekuSept Aktiv®, BIB Forte®, Alprojet W®, Desnet®, Sanihygiene®, Incidin®, D125®, Lonzagard®, and Glutishield® were tested. Time-kill assay (suspension test) was performed against six indicator bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella Typhi, Bacillus cereus, and Mycobacterium fortuitum). Low and high inoculum (final concentrations 1.5X10(6) and 9X10(6) cfu/ml) of the first five bacteria while only low level of M. fortuitum was tested. RESULTS: Cidex® (2.4% Glutaraldehyde) performed best as high level disinfectant while newer quarternary ammonium compounds (QACs) (Incidin®, D125®, and Lonzagard®) were good at low level disinfection. Sanidex® (0.55% Ortho-pthalaldehyde) though mycobactericidal took 10 minutes for sporicidal activity. Older QAC containing BIB Forte® and Desnet® took 20 minutes to fully inhibit P. aeruginosa. All disinfectants effectively reduced S. Typhi to zero counts within 5 minutes. CONCLUSION: Cidex® is a good high-level disinfectant while newer QACs (Incidin®, D125®, and Lonzagard®) were capable low-level disinfectants.
INTRODUCTION: Disinfection process validation is mandatory before introduction of a new disinfectant in hospital services. Commercial disinfection brands often question existing hospital policy claiming greater efficacy and lack of toxicity of their products. Inadvertent inadequate disinfection leads to morbidity, patient's economic burden, and the risk of mortality. AIM: To evaluate commercial disinfectants for high, intermediate and low-level disinfection so as to identify utility for our routine situations. MATERIALS AND METHODS: This laboratory based experiment was conducted at St Stephen Hospital, Delhi during July-September 2013. Twelve commercial disinfectants: Sanidex®, Sanocid®, Cidex®, SekuSept Aktiv®, BIB Forte®, Alprojet W®, Desnet®, Sanihygiene®, Incidin®, D125®, Lonzagard®, and Glutishield® were tested. Time-kill assay (suspension test) was performed against six indicator bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella Typhi, Bacillus cereus, and Mycobacterium fortuitum). Low and high inoculum (final concentrations 1.5X10(6) and 9X10(6) cfu/ml) of the first five bacteria while only low level of M. fortuitum was tested. RESULTS:Cidex® (2.4% Glutaraldehyde) performed best as high level disinfectant while newer quarternary ammonium compounds (QACs) (Incidin®, D125®, and Lonzagard®) were good at low level disinfection. Sanidex® (0.55% Ortho-pthalaldehyde) though mycobactericidal took 10 minutes for sporicidal activity. Older QAC containing BIB Forte® and Desnet® took 20 minutes to fully inhibit P. aeruginosa. All disinfectants effectively reduced S. Typhi to zero counts within 5 minutes. CONCLUSION:Cidex® is a good high-level disinfectant while newer QACs (Incidin®, D125®, and Lonzagard®) were capable low-level disinfectants.
Entities:
Keywords:
Bacillus cereus; Glutaraldehyde; High level disinfection; Mycobactericidal; Time Kill Assay
Authors: L Morales-Fernández; M Fernández-Crehuet; M Espigares; E Moreno; E Espigares Journal: Eur J Clin Microbiol Infect Dis Date: 2013-07-27 Impact factor: 3.267
Authors: Jordon K March; Marissa N Cohen; James M Lindsey; D A Millar; Chinn-Woan Lowe; Annette J Bunnell; Kim L O'Neill; G Bruce Schaalje; Richard A Robison Journal: Microbiologyopen Date: 2012-10-11 Impact factor: 3.139
Authors: Liang Li; Jinshan Jin; Haijing Hu; Ian F Deveau; Steven L Foley; Huizhong Chen Journal: J Ind Microbiol Biotechnol Date: 2022-07-30 Impact factor: 4.258