Literature DB >> 11566892

The structure of an AspRS-tRNA(Asp) complex reveals a tRNA-dependent control mechanism.

L Moulinier1, S Eiler, G Eriani, J Gangloff, J C Thierry, K Gabriel, W H McClain, D Moras.   

Abstract

The 2.6 A resolution crystal structure of an inactive complex between yeast tRNA(Asp) and Escherichia coli aspartyl-tRNA synthetase reveals the molecular details of a tRNA-induced mechanism that controls the specificity of the reaction. The dimer is asymmetric, with only one of the two bound tRNAs entering the active site cleft of its subunit. However, the flipping loop, which controls the proper positioning of the amino acid substrate, acts as a lid and prevents the correct positioning of the terminal adenosine. The structure suggests that the acceptor stem regulates the loop movement through sugar phosphate backbone- protein interactions. Solution and cellular studies on mutant tRNAs confirm the crucial role of the tRNA three-dimensional structure versus a specific recognition of bases in the control mechanism.

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Year:  2001        PMID： 11566892      PMCID： PMC125622          DOI： 10.1093/emboj/20.18.5290

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  28 in total

Review 1.  The G x U wobble base pair. A fundamental building block of RNA structure crucial to RNA function in diverse biological systems.

Authors:  G Varani; W H McClain
Journal:  EMBO Rep       Date:  2000-07       Impact factor: 8.807

2.  Plasmid systems to study RNA function in Escherichia coli.

Authors:  K Gabriel; W H McClain
Journal:  J Mol Biol       Date:  2001-07-13       Impact factor: 5.469

3.  Identity elements for specific aminoacylation of yeast tRNA(Asp) by cognate aspartyl-tRNA synthetase.

Authors:  J Pütz; J D Puglisi; C Florentz; R Giegé
Journal:  Science       Date:  1991-06-21       Impact factor: 47.728

4.  Construction of an Escherichia coli knockout strain for functional analysis of tRNA(Asp).

Authors:  W H McClain; K Gabriel
Journal:  J Mol Biol       Date:  2001-07-13       Impact factor: 5.469

5.  Crystal structure of aspartyl-tRNA synthetase from Pyrococcus kodakaraensis KOD: archaeon specificity and catalytic mechanism of adenylate formation.

Authors:  E Schmitt; L Moulinier; S Fujiwara; T Imanaka; J C Thierry; D Moras
Journal:  EMBO J       Date:  1998-09-01       Impact factor: 11.598

6.  Fast purification of a functional elongator tRNAmet expressed from a synthetic gene in vivo.

Authors:  T Meinnel; Y Mechulam; G Fayat
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

7.  Translational efficiency of transfer RNA's: uses of an extended anticodon.

Authors:  M Yarus
Journal:  Science       Date:  1982-11-12       Impact factor: 47.728

8.  Synthesis of aspartyl-tRNA(Asp) in Escherichia coli--a snapshot of the second step.

Authors:  S Eiler; A Dock-Bregeon; L Moulinier; J C Thierry; D Moras
Journal:  EMBO J       Date:  1999-11-15       Impact factor: 11.598

9.  Formation of a catalytically active complex between tRNAAsp and aspartyl-tRNA synthetase from yeast in high concentrations of ammonium sulphate.

Authors:  R Giegé; B Lorber; J P Ebel; D Moras; J C Thierry; B Jacrot; G Zaccai
Journal:  Biochimie       Date:  1982-05       Impact factor: 4.079

10.  Role of dimerization in yeast aspartyl-tRNA synthetase and importance of the class II invariant proline.

Authors:  G Eriani; J Cavarelli; F Martin; G Dirheimer; D Moras; J Gangloff
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-15       Impact factor: 11.205
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  29 in total

Review 1.  Aminoacyl-tRNA synthetases: versatile players in the changing theater of translation.

Authors:  Christopher Francklyn; John J Perona; Joern Puetz; Ya-Ming Hou
Journal:  RNA       Date:  2002-11       Impact factor: 4.942

2.  When contemporary aminoacyl-tRNA synthetases invent their cognate amino acid metabolism.

Authors:  Hervé Roy; Hubert Dominique Becker; Joseph Reinbolt; Daniel Kern
Journal:  Proc Natl Acad Sci U S A       Date:  2003-07-21       Impact factor: 11.205

3.  A yeast knockout strain to discriminate between active and inactive tRNA molecules.

Authors:  Renaud Geslain; Franck Martin; Alain Camasses; Gilbert Eriani
Journal:  Nucleic Acids Res       Date:  2003-08-15       Impact factor: 16.971

4.  Single amino acid changes in AspRS reveal alternative routes for expanding its tRNA repertoire in vivo.

Authors:  Franck Martin; Sharief Barends; Gilbert Eriani
Journal:  Nucleic Acids Res       Date:  2004-08-02       Impact factor: 16.971

5.  Kinetic discrimination of tRNA identity by the conserved motif 2 loop of a class II aminoacyl-tRNA synthetase.

Authors:  Ethan C Guth; Christopher S Francklyn
Journal:  Mol Cell       Date:  2007-02-23       Impact factor: 17.970

6.  Recognition of acceptor-stem structure of tRNA(Asp) by Escherichia coli aspartyl-tRNA synthetase.

Authors:  Hyunsic Choi; Kay Gabriel; Jay Schneider; Sharee Otten; William H McClain
Journal:  RNA       Date:  2003-04       Impact factor: 4.942

7.  Aptamer redesigned tRNA is nonfunctional and degraded in cells.

Authors:  Dennis Lee; William H McClain
Journal:  RNA       Date:  2004-01       Impact factor: 4.942

8.  RNA-assisted catalysis in a protein enzyme: The 2'-hydroxyl of tRNA(Thr) A76 promotes aminoacylation by threonyl-tRNA synthetase.

Authors:  Anand Minajigi; Christopher S Francklyn
Journal:  Proc Natl Acad Sci U S A       Date:  2008-11-07       Impact factor: 11.205

Review 9.  DNA polymerases and aminoacyl-tRNA synthetases: shared mechanisms for ensuring the fidelity of gene expression.

Authors:  Christopher S Francklyn
Journal:  Biochemistry       Date:  2008-10-14       Impact factor: 3.162

10.  Plasmodial aspartyl-tRNA synthetases and peculiarities in Plasmodium falciparum.

Authors:  Tania Bour; Aziza Akaddar; Bernard Lorber; Sébastien Blais; Christian Balg; Ermanno Candolfi; Magali Frugier
Journal:  J Biol Chem       Date:  2009-05-14       Impact factor: 5.157
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