Literature DB >> 11330812

Measurement of one-bond 1H-13C, couplings in backbone-labelled proteins.

A W Giesen1, L C Bae, C L Barrett, J A Chyba, M M Chaykovsky, M C Cheng, J H Murray, E J Oliver, S M Sullivan, J M Brown, F W Dahlquist, S W Homans.   

Abstract

NMR dipole-dipole couplings between protein backbone nuclei (1H(alpha), 13C(alpha), 15N, 1H(N), 13C') offer enormous scope for the rapid determination of protein global folds. Here, we show that measurement of one-bond splittings in the protein backbone is facilitated by use of protein that is selectively isotopically enriched only in the backbone atoms. In particular, 1H(alpha)-13C(alpha) couplings can be measured simply and with high sensitivity by use of conventional heteronuclear single quantum correlation (HSQC) techniques.

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Year:  2001        PMID： 11330812     DOI： 10.1023/a:1011298531256

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  15 in total

1.  Residual dipolar couplings as new conformational restraints in isotropically 13C-enriched oligosaccharides.

Authors:  G R Kiddle; S W Homans
Journal:  FEBS Lett       Date:  1998-09-25       Impact factor: 4.124

2.  Multisite band-selective decoupling in proteins.

Authors:  E Kupce; G Wagner
Journal:  J Magn Reson B       Date:  1996-03

Review 3.  Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra.

Authors:  M Ottiger; F Delaglio; A Bax
Journal:  J Magn Reson       Date:  1998-04       Impact factor: 2.229

4.  Measurement of dipolar contributions to 1JCH splittings from magnetic-field dependence of J modulation in two-dimensional NMR spectra.

Authors:  N Tjandra; A Bax
Journal:  J Magn Reson       Date:  1997-02       Impact factor: 2.229

5.  Resolution and sensitivity gain in HCCH-TOCSY experiments by homonuclear C beta decoupling.

Authors:  H Matsuo; E Kupce; G Wagner
Journal:  J Magn Reson B       Date:  1996-11

6.  Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values.

Authors:  M Ottiger; A Bax
Journal:  J Biomol NMR       Date:  1999-02       Impact factor: 2.835

7.  Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions.

Authors:  M R Hansen; L Mueller; A Pardi
Journal:  Nat Struct Biol       Date:  1998-12

8.  Characterization of magnetically oriented phospholipid micelles for measurement of dipolar couplings in macromolecules.

Authors:  M Ottiger; A Bax
Journal:  J Biomol NMR       Date:  1998-10       Impact factor: 2.835

9.  Characterization of magnetically orientable bilayers in mixtures of dihexanoylphosphatidylcholine and dimyristoylphosphatidylcholine by solid-state NMR.

Authors:  C R Sanders; J P Schwonek
Journal:  Biochemistry       Date:  1992-09-22       Impact factor: 3.162

10.  Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.

Authors:  J R Tolman; J M Flanagan; M A Kennedy; J H Prestegard
Journal:  Proc Natl Acad Sci U S A       Date:  1995-09-26       Impact factor: 11.205
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  4 in total

1.  High-resolution NMR spectroscopy of a GPCR in aligned bicelles.

Authors:  Sang Ho Park; Stefan Prytulla; Anna A De Angelis; Jonathan Miles Brown; Hans Kiefer; Stanley J Opella
Journal:  J Am Chem Soc       Date:  2006-06-14       Impact factor: 15.419

2.  Simultaneous alpha/beta spin-state selection for (13)C and (15)N from a time-shared HSQC-IPAP experiment.

Authors:  Pau Nolis; Teodor Parella
Journal:  J Biomol NMR       Date:  2006-12-12       Impact factor: 2.835

3.  Triple resonance experiments for aligned sample solid-state NMR of (13)C and (15)N labeled proteins.

Authors:  Neeraj Sinha; Christopher V Grant; Sang Ho Park; Jonathan Miles Brown; Stanley J Opella
Journal:  J Magn Reson       Date:  2007-01-24       Impact factor: 2.229

4.  1H-filtered correlation experiments for assignment and determination of coupling constants in backbone labelled proteins.

Authors:  Alexander W Giesen; Lynnette C Bae; Christa L Barrett; Jason A Chyba; Michael M Chaykovsky; Minn-Chang Cheng; Jenny H Murray; Ed J Oliver; Sarah M Sullivan; Jonathan Miles Brown; Steve W Homans
Journal:  J Biomol NMR       Date:  2002-01       Impact factor: 2.835
  4 in total

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