Literature DB >> 11208811

Experimental evolution of enzyme temperature activity profile: selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase.

M Roovers1, R Sanchez, C Legrain, N Glansdorff.   

Abstract

We have obtained mutants of Pyrococcus furiosus ornithine carbamoyltransferase active at low temperatures by selecting for complementation of an appropriate yeast mutant after in vivo mutagenesis. The mutants were double ones, still complementing at 15 degrees C, a temperature already in the psychrophilic range. Their kinetic analysis is reported.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11208811      PMCID： PMC94980          DOI： 10.1128/JB.183.3.1101-1105.2001

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  23 in total

1.  Ornithine carbamoyltransferase from Pyrococcus furiosus.

Authors:  C Legrain; V Villeret; M Roovers; C Tricot; B Clantin; J Van Beeumen; V Stalon; N Glansdorff
Journal:  Methods Enzymol       Date:  2001       Impact factor: 1.600

Review 2.  Psychrophilic enzymes: molecular basis of cold adaptation.

Authors:  G Feller; C Gerday
Journal:  Cell Mol Life Sci       Date:  1997-10       Impact factor: 9.261

Review 3.  Adaptive mutation: the uses of adversity.

Authors:  P L Foster
Journal:  Annu Rev Microbiol       Date:  1993       Impact factor: 15.500

4.  Cold adaptation of proteins. Purification, characterization, and sequence of the heat-labile subtilisin from the antarctic psychrophile Bacillus TA41.

Authors:  S Davail; G Feller; E Narinx; C Gerday
Journal:  J Biol Chem       Date:  1994-07-01       Impact factor: 5.157

5.  Isolation of a thermostable enzyme variant by cloning and selection in a thermophile.

Authors:  H Liao; T McKenzie; R Hageman
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

6.  Enzymes from cold-adapted microorganisms. The class C beta-lactamase from the antarctic psychrophile Psychrobacter immobilis A5.

Authors:  G Feller; Z Zekhnini; J Lamotte-Brasseur; C Gerday
Journal:  Eur J Biochem       Date:  1997-02-15

7.  Biochemical characterisation of ornithine carbamoyltransferase from Pyrococcus furiosus.

Authors:  C Legrain; V Villeret; M Roovers; D Gigot; O Dideberg; A Piérard; N Glansdorff
Journal:  Eur J Biochem       Date:  1997-08-01

8.  Isolation of the gene encoding Pyrococcus furiosus ornithine carbamoyltransferase and study of its expression profile in vivo and in vitro.

Authors:  M Roovers; C Hethke; C Legrain; M Thomm; N Glansdorff
Journal:  Eur J Biochem       Date:  1997-08-01

9.  Crystal structure of Pseudomonas aeruginosa catabolic ornithine transcarbamoylase at 3.0-A resolution: a different oligomeric organization in the transcarbamoylase family.

Authors:  V Villeret; C Tricot; V Stalon; O Dideberg
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-07       Impact factor: 11.205

10.  Screening of stable proteins in an extreme thermophile, Thermus thermophilus.

Authors:  M Tamakoshi; A Yamagishi; T Oshima
Journal:  Mol Microbiol       Date:  1995-06       Impact factor: 3.501
View more
  6 in total

Review 1.  Molecular basis of cold adaptation.

Authors:  Salvino D'Amico; Paule Claverie; Tony Collins; Daphné Georlette; Emmanuelle Gratia; Anne Hoyoux; Marie-Alice Meuwis; Georges Feller; Charles Gerday
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2002-07-29       Impact factor: 6.237

2.  Improved thermal stability and activity in the cold-adapted lipase B from Candida antarctica following chemical modification with oxidized polysaccharides.

Authors:  Khawar Sohail Siddiqui; Ricardo Cavicchioli
Journal:  Extremophiles       Date:  2005-07-06       Impact factor: 2.395

3.  DNA family shuffling of hyperthermostable beta-glycosidases.

Authors:  Thijs Kaper; Stan J J Brouns; Ans C M Geerling; Willem M De Vos; John Van der Oost
Journal:  Biochem J       Date:  2002-12-01       Impact factor: 3.857

4.  Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in ornithine carbamoyltransferase from Moritella abyssi.

Authors:  Ying Xu; Georges Feller; Charles Gerday; Nicolas Glansdorff
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

5.  Temperature dependent mistranslation in a hyperthermophile adapts proteins to lower temperatures.

Authors:  Michael H Schwartz; Tao Pan
Journal:  Nucleic Acids Res       Date:  2015-12-10       Impact factor: 16.971

6.  Enzymology of the pathway for ATP production by arginine breakdown.

Authors:  Tjeerd Pols; Shubham Singh; Cecile Deelman-Driessen; Bauke F Gaastra; Bert Poolman
Journal:  FEBS J       Date:  2020-05-05       Impact factor: 5.542
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.