Literature DB >> 1094460

Cloning, isolation, and characterization of replication regions of complex plasmid genomes.

K Timmis, F Cabello, S N Cohen.   

Abstract

EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras and the parent replicons has localized the cloned R6-5 replication region to a DNA segment between kilobase pair coordinates 1.0 and 88.0 on the R6-5 map. Physical proximity between the plasmid replication functions and the locus governing plasmid incompatibility has been shown for both parent replicons. The cloning method reported appears to be generally applicable for the identification and isolation of replication regions of a variety of complex genomes.

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Year:  1975        PMID： 1094460      PMCID： PMC432733          DOI： 10.1073/pnas.72.6.2242

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Construction of biologically functional bacterial plasmids in vitro.

Authors:  S N Cohen; A C Chang; H W Boyer; R B Helling
Journal:  Proc Natl Acad Sci U S A       Date:  1973-11       Impact factor: 11.205

2.  Replication of colicin E1 plasmid DNA in cell extracts.

Authors:  Y Sakakibara; J I Tomizawa
Journal:  Proc Natl Acad Sci U S A       Date:  1974-03       Impact factor: 11.205

3.  Identification and mapping of the replication genes of an R factor, R100-1, integrated into the chromosome of Escherichia coli K-12.

Authors:  M Yoshikawa
Journal:  J Bacteriol       Date:  1974-06       Impact factor: 3.490

Review 4.  Pedigrees of some mutant strains of Escherichia coli K-12.

Authors:  B J Bachmann
Journal:  Bacteriol Rev       Date:  1972-12

5.  Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.

Authors:  P A Sharp; M T Hsu; E Otsubo; N Davidson
Journal:  J Mol Biol       Date:  1972-11-14       Impact factor: 5.469

6.  Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors.

Authors:  P A Sharp; S N Cohen; N Davidson
Journal:  J Mol Biol       Date:  1973-04-05       Impact factor: 5.469

7.  Nonchromosomal antibiotic resistance in bacteria. V. Isolation and characterization of R factor mutants exhibiting temperature-sensitive repression of fertility.

Authors:  R P Silver; S N Cohen
Journal:  J Bacteriol       Date:  1972-06       Impact factor: 3.490

8.  Repressor-minus and operator-constitutive de-repressed mutants of F-like R factors: their effect on chromosomal transfer by HfrC.

Authors:  E Meynell; M Cooke
Journal:  Genet Res       Date:  1969-12       Impact factor: 1.588

9.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

10.  Genome construction between bacterial species in vitro: replication and expression of Staphylococcus plasmid genes in Escherichia coli.

Authors:  A C Chang; S N Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205
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  125 in total

1.  Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

Authors:  R A Skurray; H Nagaishi; A J Clark
Journal:  Proc Natl Acad Sci U S A       Date:  1976-01       Impact factor: 11.205

Review 2.  In search of the holy replicator.

Authors:  David M Gilbert
Journal:  Nat Rev Mol Cell Biol       Date:  2004-10       Impact factor: 94.444

3.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors:  A C Chang; S N Cohen
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

4.  Generation of miniplasmids from copy number mutants of the R plasmid NR1.

Authors:  D P Taylor; J Greenberg; R H Rownd
Journal:  J Bacteriol       Date:  1977-12       Impact factor: 3.490

5.  Purification and characterization of the sopB gene product which is responsible for stable maintenance of mini-F plasmid.

Authors:  E Watanabe; S Inamoto; M H Lee; S U Kim; T Ogua; H Mori; S Hiraga; M Yamasaki; K Nagai
Journal:  Mol Gen Genet       Date:  1989-09

Review 6.  Beta-lactamase plasmids and chromosomally mediated antibiotic resistance in pathogenic Neisseria species.

Authors:  J A Dillon; K H Yeung
Journal:  Clin Microbiol Rev       Date:  1989-04       Impact factor: 26.132

7.  Propagation of satellite phage P4 as a plasmid.

Authors:  R Goldstein; J Sedivy; E Ljungquist
Journal:  Proc Natl Acad Sci U S A       Date:  1982-01       Impact factor: 11.205

8.  Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2.

Authors:  R Meyer; D Figurski; D R Helinski
Journal:  Mol Gen Genet       Date:  1977-04-29

9.  Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.

Authors:  F C Franklin; M Bagdasarian; M M Bagdasarian; K N Timmis
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

10.  Restriction endonuclease mapping and mutagenesis of the F sex factor replication region.

Authors:  J J Manis; B C Kline
Journal:  Mol Gen Genet       Date:  1977-04-29
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