Literature DB >> 327269

Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2.

R Meyer, D Figurski, D R Helinski.   

Abstract

The cleavage map of the plasmid RK2 was determined for the five restriction endonucleases EcoRI, HindIII, BamH-I, SalI and HpaI. DNA has been inserted into several of these sites and cloned in Escherichia coli. Efforts to obtain derivatives of RK2 reduced in size by restriction endonuclease digestion of the plasmid were not successful and indicated that genes required for the maintenance of this plasmid in E. coli are not tightly clustered. An RK2 derivative possessing an internal molecular rearrangement was obtained by transformation with restriction endonuclease digests of the plasmid.

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Year:  1977        PMID： 327269     DOI： 10.1007/bf00268809

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  18 in total

1.  Cloning, isolation, and characterization of replication regions of complex plasmid genomes.

Authors:  K Timmis; F Cabello; S N Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

2.  Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H.

Authors:  G A Wilson; F E Young
Journal:  J Mol Biol       Date:  1975-09-05       Impact factor: 5.469

3.  Host range and properties of the Pseudomonas aeruginosa R factor R1822.

Authors:  R H Olsen; P Shipley
Journal:  J Bacteriol       Date:  1973-02       Impact factor: 3.490

4.  Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli.

Authors:  M Bazaral; D R Helinski
Journal:  J Mol Biol       Date:  1968-09-14       Impact factor: 5.469

5.  Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI.

Authors:  D H Hamer; C A Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

6.  Cleavage of Simian virus 40 DNA at a unique site by a bacterial restriction enzyme.

Authors:  J F Morrow; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1972-11       Impact factor: 11.205

7.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

8.  Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.

Authors:  M A Lovett; D R Helinski
Journal:  J Bacteriol       Date:  1976-08       Impact factor: 3.490

9.  Molecular vehicle properties of the broad host range plasmid RK2.

Authors:  R Meyer; D Figurski; D R Helinski
Journal:  Science       Date:  1975-12-19       Impact factor: 47.728

10.  Action of haemophilus endodeoxyribonuclease on biologically active deoxyribonucleic acid.

Authors:  R Gromkova; S H Goodgal
Journal:  J Bacteriol       Date:  1972-03       Impact factor: 3.490
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  47 in total

1.  Structural, molecular, and genetic analysis of the kilA operon of broad-host-range plasmid RK2.

Authors:  P Goncharoff; S Saadi; C H Chang; L H Saltman; D H Figurski
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

2.  Isolation and Characterization of Marine Caulobacters and Assessment of Their Potential for Genetic Experimentation.

Authors:  Nick Anast; John Smit
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792

3.  Transposon mutagenesis in a marine synechococcus strain: isolation of swimming motility mutants.

Authors:  J McCarren; B Brahamsha
Journal:  J Bacteriol       Date:  2005-07       Impact factor: 3.490

4.  Replication control in promiscuous plasmid RK2: kil and kor functions affect expression of the essential replication gene trfA.

Authors:  H C Schreiner; D H Bechhofer; R F Pohlman; C Young; P A Borden; D H Figurski
Journal:  J Bacteriol       Date:  1985-07       Impact factor: 3.490

5.  Broad host range plasmid RK2 encodes multiple kil genes potentially lethal to Escherichia coli host cells.

Authors:  D H Figurski; R F Pohlman; D H Bechhofer; A S Prince; C A Kelton
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

6.  Regions of broad-host-range plasmid RK2 involved in replication and stable maintenance in nine species of gram-negative bacteria.

Authors:  T J Schmidhauser; D R Helinski
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

7.  Transcriptional analysis, translational analysis, and sequence of the kilA-tellurite resistance region of plasmid RK2Ter.

Authors:  E G Walter; C M Thomas; J P Ibbotson; D E Taylor
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

8.  Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.

Authors:  D H Figurski; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

9.  Transcription in the trfA region of broad host range plasmid RK2 is regulated by trfB and korB.

Authors:  V Shinger; C M Thomas
Journal:  Mol Gen Genet       Date:  1984

10.  Novel system for recognizing and eliminating foreign DNA in Pseudomonas putida.

Authors:  K Kim; R J Meyer
Journal:  J Bacteriol       Date:  1984-08       Impact factor: 3.490
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