Literature DB >> 1108020

Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

R A Skurray, H Nagaishi, A J Clark.   

Abstract

We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of EcoRI digests and were arranged in a series of overlapping fragments using the EcoRI fragment map of F established by H. Ohtsubo and E. Ohtsubo. The clones isolated demonstrate one or more of the following F-specified functions: inhibition of female-specific phage (T7) multiplication, formation of F pili, surface exclusion, or immunity to lethal zygosis. These properties are discussed in terms of the EcoRI fragments of F that specify them.

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Year:  1976        PMID： 1108020      PMCID： PMC335839          DOI： 10.1073/pnas.73.1.64

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  36 in total

1.  Operon structure of DNA transfer cistrons on the F sex factor.

Authors:  R Helmuth; M Achtman
Journal:  Nature       Date:  1975-10-23       Impact factor: 49.962

2.  Genetic analysis of a "double male" strain of Escherichia coli K-12.

Authors:  A J CLARK
Journal:  Genetics       Date:  1963-01       Impact factor: 4.562

3.  [Processes of conjugation and recombination in Escherichia coli. I. Induction by conjugation or zygotic induction].

Authors:  F JACOB; E L WOLLMAN
Journal:  Ann Inst Pasteur (Paris)       Date:  1956-10

4.  Sex Compatibility in Escherichia Coli.

Authors:  J Lederberg; L L Cavalli; E M Lederberg
Journal:  Genetics       Date:  1952-11       Impact factor: 4.562

5.  Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends.

Authors:  J E Mertz; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1972-11       Impact factor: 11.205

6.  Mutants of Escherichia coli with altered deoxyribonucleases. I. Isolation and characterization of mutants for exonuclease 3.

Authors:  C Milcarek; B Weiss
Journal:  J Mol Biol       Date:  1972-07-21       Impact factor: 5.469

7.  Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

Authors:  R B Helling; H M Goodman; H W Boyer
Journal:  J Virol       Date:  1974-11       Impact factor: 5.103

Review 8.  Extrachromosomal inheritance in bacteria.

Authors:  R P Novick
Journal:  Bacteriol Rev       Date:  1969-06

9.  T7 translational control mechanisms and their inhibiton by F factors.

Authors:  T G Morrison; M H Malamy
Journal:  Nat New Biol       Date:  1971-05-12

10.  Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.

Authors:  V Hershfield; H W Boyer; C Yanofsky; M A Lovett; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205
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  64 in total

1.  Comparison of proteins involved in pilus synthesis and mating pair stabilization from the related plasmids F and R100-1: insights into the mechanism of conjugation.

Authors:  K G Anthony; W A Klimke; J Manchak; L S Frost
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

Review 2.  TraT lipoprotein, a plasmid-specified mediator of interactions between gram-negative bacteria and their environment.

Authors:  S Sukupolvi; C D O'Connor
Journal:  Microbiol Rev       Date:  1990-12

3.  Structure and function of conjugative pili: monoclonal antibodies as probes for structural variants of F pili.

Authors:  T H Grossman; L S Frost; P M Silverman
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

4.  traY and traI are required for oriT-dependent enhanced recombination between lac-containing plasmids and lambda plac5.

Authors:  J R Carter; R D Porter
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

5.  Transposon 10 promoted deletions and inversions in the transfer genes of R100-1.

Authors:  M A Kehoe; T J Foster
Journal:  Mol Gen Genet       Date:  1979-10-02

6.  Restriction endonuclease mapping and mutagenesis of the F sex factor replication region.

Authors:  J J Manis; B C Kline
Journal:  Mol Gen Genet       Date:  1977-04-29

7.  Plasmid replication functions. II. Cloning analysis of the repA replication region of antibiotic resistance plasmid R6-5.

Authors:  I Andrés; P M Slocombe; F Cabello; J K Timmis; R Lurz; H J Burkardt; K N Timmis
Journal:  Mol Gen Genet       Date:  1979-01-05

8.  Origin of replication, oriC, or the Escherichia coli chromosome on specialized transducing phages lambda asn.

Authors:  K von Meyenburg; F G Hansen; L D Nielsin; E Riise
Journal:  Mol Gen Genet       Date:  1978-04-17

9.  Characterization of an Escherichia coli plasmid encoding for synthesis of heat-labile toxin: molecular cloning of the toxin determinant.

Authors:  M So; W S Dallas; S Falkow
Journal:  Infect Immun       Date:  1978-08       Impact factor: 3.441

10.  Low molecular weight RNA species encoded by a multiple drug resistance plasmid.

Authors:  M De Wilde; J E Davies; F J Schmidt
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205
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