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Literature DB >> 10908354

Homogeneous assays based on deoxyribozyme catalysis.

M N Stojanovic¹, P de Prada, D W Landry.

Abstract

We report herein the first homogeneous assays based on the ribonuclease activity of a deoxyribozyme. The previously reported deoxyribozyme was covalently modified with biotin and used to assay biotin-binding interactions through changes in fluorescence upon substrate turnover. Deoxyribozymes with fluorescence-based reporting have the potential to serve as general analytical tools.

Entities: Chemical

Mesh：

Substances：

Year: 2000 PMID： 10908354 PMCID： PMC102685 DOI： 10.1093/nar/28.15.2915

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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