R F Brubaker1, W M Bourne, L A Bachman, J W McLaren. 1. Department of Ophthalmology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA. brubaker.richard@mayo.edu
Abstract
PURPOSE: To measure the concentration of ascorbic acid in the human corneal epithelium. METHODS: Corneal epithelium was removed from postmortem eyes 4 to 16 hours after death and ascorbate measured by high-performance liquid chromatography. RESULTS: The concentration of ascorbate was 1.33 +/- 0.48 mg/gm wet weight (mean +/- SD), estimated to be 14 times its concentration in the aqueous humor. CONCLUSIONS: Ascorbate can protect the basal layer of the epithelium by absorption of incident ultraviolet radiation.
PURPOSE: To measure the concentration of ascorbic acid in the human corneal epithelium. METHODS: Corneal epithelium was removed from postmortem eyes 4 to 16 hours after death and ascorbate measured by high-performance liquid chromatography. RESULTS: The concentration of ascorbate was 1.33 +/- 0.48 mg/gm wet weight (mean +/- SD), estimated to be 14 times its concentration in the aqueous humor. CONCLUSIONS:Ascorbate can protect the basal layer of the epithelium by absorption of incident ultraviolet radiation.
Authors: Mukoma F Simpanya; Graeme Wistow; James Gao; Larry L David; Frank J Giblin; Kenneth P Mitton Journal: Mol Vis Date: 2008-12-19 Impact factor: 2.367