P Zafrilla1, M Losada, A Perez, G Caravaca, J Mulero. 1. Department of Food Technology and Nutrition, Catholic University of San Antonio, Murcia 30107, Spain. mpzafrilla@ucam.edu
Abstract
OBJECTIVE: The aim of this study was to analyze biomarkers of oxidative stress in patients with wet age related macular degeneration (AMD). PARTICIPANTS AND MEASUREMENTS: Case-control study that includes 163 patients with wet AMD (age group of 55-82 years with the mean age of 71 years and 170 age-matched healthy controls in the age group of 55-78 years with the mean age of 71 years. The following parameters were determined: reduced and oxidized Glutathione (GSH/GSSH), protein carbonyl groups, total antioxidant activity in plasma and the activity of endogenous antioxidant enzymes, such as, gluthatione peroxidase, gluthatione reductase and superoxide dismutase. RESULTS: We observed total antioxidant activity higher in control group (CG) compared with patients with wet AMD (7.1 ± 1.2 μM Trolox vs 5.8 ± 1.1 μM Trolox). Values of superoxide dismutase activity (SOD), gluthatione reductase (GR) and gluthatione peroxidase (GPx) are higher in control group than in patients with wet AMD. According to the GSH/GSSH results, average values were higher in the CG than in patients with wet AMD and data were not significantly different.. Values of protein carbonyl groups were higher in patients with wet AMD than in CG and significant differences were found. CONCLUSIONS: The finding of the present study suggests that the patients with wet AMD are an altered metabolic state of oxidation-reduction and that it is convenient to give therapeutic interventions with antioxidants. We have demonstrated that systematic oxidative stress, measured by different biomarkers is closely associated with the wet AMD.
OBJECTIVE: The aim of this study was to analyze biomarkers of oxidative stress in patients with wet age related macular degeneration (AMD). PARTICIPANTS AND MEASUREMENTS: Case-control study that includes 163 patients with wet AMD (age group of 55-82 years with the mean age of 71 years and 170 age-matched healthy controls in the age group of 55-78 years with the mean age of 71 years. The following parameters were determined: reduced and oxidized Glutathione (GSH/GSSH), protein carbonyl groups, total antioxidant activity in plasma and the activity of endogenous antioxidant enzymes, such as, gluthatione peroxidase, gluthatione reductase and superoxide dismutase. RESULTS: We observed total antioxidant activity higher in control group (CG) compared with patients with wet AMD (7.1 ± 1.2 μM Trolox vs 5.8 ± 1.1 μM Trolox). Values of superoxide dismutase activity (SOD), gluthatione reductase (GR) and gluthatione peroxidase (GPx) are higher in control group than in patients with wet AMD. According to the GSH/GSSH results, average values were higher in the CG than in patients with wet AMD and data were not significantly different.. Values of protein carbonyl groups were higher in patients with wet AMD than in CG and significant differences were found. CONCLUSIONS: The finding of the present study suggests that the patients with wet AMD are an altered metabolic state of oxidation-reduction and that it is convenient to give therapeutic interventions with antioxidants. We have demonstrated that systematic oxidative stress, measured by different biomarkers is closely associated with the wet AMD.
Authors: P S Samiec; C Drews-Botsch; E W Flagg; J C Kurtz; P Sternberg; R L Reed; D P Jones Journal: Free Radic Biol Med Date: 1998-03-15 Impact factor: 7.376
Authors: Cem Evereklioglu; Hamdi Er; Selim Doganay; Mustafa Cekmen; Yusuf Turkoz; Baris Otlu; Elif Ozerol Journal: Doc Ophthalmol Date: 2003-03 Impact factor: 2.379
Authors: Jin Yang; Yao Li; Lawrence Chan; Yi-Ting Tsai; Wen-Hsuan Wu; Huy V Nguyen; Chun-Wei Hsu; Xiaorong Li; Lewis M Brown; Dieter Egli; Janet R Sparrow; Stephen H Tsang Journal: Hum Mol Genet Date: 2014-02-04 Impact factor: 6.150
Authors: Jordano Ferreira Reis; Valter Vinicius Silva Monteiro; Rafaelli de Souza Gomes; Matheus Moraes do Carmo; Glauber Vilhena da Costa; Paula Cardoso Ribera; Marta Chagas Monteiro Journal: J Transl Med Date: 2016-11-15 Impact factor: 5.531
Authors: Dorota Rogińska; Miłosz P Kawa; Ewa Pius-Sadowska; Renata Lejkowska; Karolina Łuczkowska; Barbara Wiszniewska; Kai Kaarniranta; Jussi J Paterno; Christian A Schmidt; Bogusław Machaliński; Anna Machalińska Journal: Oxid Med Cell Longev Date: 2017-08-08 Impact factor: 6.543
Authors: Martha P Montgomery; Eric Postel; David M Umbach; Marie Richards; Mary Watson; Aaron Blair; Honglei Chen; Dale P Sandler; Silke Schmidt; Freya Kamel Journal: Environ Health Perspect Date: 2017-07-19 Impact factor: 9.031