Literature DB >> 10818357

Crystallization and initial crystallographic analysis of phosphomannomutase/phosphoglucomutase from Pseudomonas aeruginosa.

C A Regni1, P A Tipton, L J Beamer.   

Abstract

The enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) catalyzes the conversion of mannose 6-phosphate to mannose 1-phosphate in the second step of the alginate biosynthetic pathway of Pseudomonas aeruginosa. PMM/PGM has been crystallized by hanging-drop vapor diffusion in space group P2(1)2(1)2(1). Crystals diffract to 1.75 A resolution on a synchrotron X-ray source under cryo-cooling conditions. PMM/PGM substituted with selenomethionine has been purified and crystallizes isomorphously with the native enzyme. Structure determination by MAD phasing is under way. Because of its role in alginate biosynthesis, PMM/PGM is a potential target for therapeutic inhibitors to combat P. aeruginosa infections.

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Year:  2000        PMID： 10818357     DOI： 10.1107/s0907444900004431

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  9 in total

1.  Breaking the covalent connection: Chain connectivity and the catalytic reaction of PMM/PGM.

Authors:  Andrew M Schramm; Dale Karr; Ritcha Mehra-Chaudhary; Steven R Van Doren; Cristina M Furdui; Lesa J Beamer
Journal:  Protein Sci       Date:  2010-06       Impact factor: 6.725

2.  Complexes of the enzyme phosphomannomutase/phosphoglucomutase with a slow substrate and an inhibitor.

Authors:  Catherine Regni; Grant S Shackelford; Lesa J Beamer
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-07-24

3.  Epimerase active domain of Pseudomonas aeruginosa AlgG, a protein that contains a right-handed beta-helix.

Authors:  Stephanie A Douthit; Mensur Dlakic; Dennis E Ohman; Michael J Franklin
Journal:  J Bacteriol       Date:  2005-07       Impact factor: 3.490

4.  Promotion of enzyme flexibility by dephosphorylation and coupling to the catalytic mechanism of a phosphohexomutase.

Authors:  Yingying Lee; Maria T Villar; Antonio Artigues; Lesa J Beamer
Journal:  J Biol Chem       Date:  2014-01-08       Impact factor: 5.157

5.  Solution NMR of a 463-residue phosphohexomutase: domain 4 mobility, substates, and phosphoryl transfer defect.

Authors:  Akella V S Sarma; Asokan Anbanandam; Allek Kelm; Ritcha Mehra-Chaudhary; Yirui Wei; Peiwu Qin; Yingying Lee; Mark V Berjanskii; Jacob A Mick; Lesa J Beamer; Steven R Van Doren
Journal:  Biochemistry       Date:  2012-01-17       Impact factor: 3.162

6.  Crystal structure of a bacterial phosphoglucomutase, an enzyme involved in the virulence of multiple human pathogens.

Authors:  Ritcha Mehra-Chaudhary; Jacob Mick; John J Tanner; Michael T Henzl; Lesa J Beamer
Journal:  Proteins       Date:  2011-01-18

7.  Genetics of bacterial alginate: alginate genes distribution, organization and biosynthesis in bacteria.

Authors:  Nuzhat Ahmed
Journal:  Curr Genomics       Date:  2007-05       Impact factor: 2.236

8.  A coevolutionary residue network at the site of a functionally important conformational change in a phosphohexomutase enzyme family.

Authors:  Yingying Lee; Jacob Mick; Cristina Furdui; Lesa J Beamer
Journal:  PLoS One       Date:  2012-06-07       Impact factor: 3.240

9.  Multiple Ligand-Bound States of a Phosphohexomutase Revealed by Principal Component Analysis of NMR Peak Shifts.

Authors:  Jia Xu; Akella V S Sarma; Yirui Wei; Lesa J Beamer; Steven R Van Doren
Journal:  Sci Rep       Date:  2017-07-13       Impact factor: 4.379
  9 in total

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