Literature DB >> 10773094

Alkaline fixation drastically improves the signal of in situ hybridization.

E Basyuk1, E Bertrand, L Journot.   

Abstract

In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the influence of the pH of the fixative on the outcome of ISH. Our studies indicate that alkaline formaldehyde dramatically increases the ISH signal with RNA probes. The increase in signal was observed for detection of low as well as for high abundance messages. The sensitivity of the method was increased 5- to 6-fold.

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Year:  2000        PMID： 10773094      PMCID： PMC105385          DOI： 10.1093/nar/28.10.e46

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

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Authors:  F Bian; T Chu; K Schilling; J Oberdick
Journal:  Mol Cell Neurosci       Date:  1996-02       Impact factor: 4.314

2.  Formation and detection of RNA-DNA hybrid molecules in cytological preparations.

Authors:  J G Gall; M L Pardue
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3.  RNA-DNA hybrids at the cytological level.

Authors:  H A John; M L Birnstiel; K W Jones
Journal:  Nature       Date:  1969-08-09       Impact factor: 49.962

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Authors:  L J Karr; A Panoskaltsis-Mortari; J Li; D Devore-Carter; C T Weaver; R P Bucy
Journal:  J Immunol Methods       Date:  1995-05-11       Impact factor: 2.303

5.  Improved design of riboprobes from pBluescript and related vectors for in situ hybridization.

Authors:  H Witkiewicz; M E Bolander; D R Edwards
Journal:  Biotechniques       Date:  1993-03       Impact factor: 1.993

6.  Loss of expression of the candidate tumor suppressor gene ZAC in breast cancer cell lines and primary tumors.

Authors:  B Bilanges; A Varrault; E Basyuk; C Rodriguez; A Mazumdar; C Pantaloni; J Bockaert; C Theillet; D Spengler; L Journot
Journal:  Oncogene       Date:  1999-07-08       Impact factor: 9.867

7.  A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes.

Authors:  N Schaeren-Wiemers; A Gerfin-Moser
Journal:  Histochemistry       Date:  1993-12

8.  hZAC encodes a zinc finger protein with antiproliferative properties and maps to a chromosomal region frequently lost in cancer.

Authors:  A Varrault; E Ciani; F Apiou; B Bilanges; A Hoffmann; C Pantaloni; J Bockaert; D Spengler; L Journot
Journal:  Proc Natl Acad Sci U S A       Date:  1998-07-21       Impact factor: 11.205

9.  Poly(A) RNA codistribution with microfilaments: evaluation by in situ hybridization and quantitative digital imaging microscopy.

Authors:  K L Taneja; L M Lifshitz; F S Fay; R H Singer
Journal:  J Cell Biol       Date:  1992-12       Impact factor: 10.539
  9 in total
  9 in total

Review 1.  Effect of fixatives and tissue processing on the content and integrity of nucleic acids.

Authors:  Mythily Srinivasan; Daniel Sedmak; Scott Jewell
Journal:  Am J Pathol       Date:  2002-12       Impact factor: 4.307

2.  Conversions of formaldehyde-modified 2'-deoxyadenosine 5'-monophosphate in conditions modeling formalin-fixed tissue dehydration.

Authors:  Vladimir K Rait; Qingrong Zhang; Daniele Fabris; Jeffrey T Mason; Timothy J O'Leary
Journal:  J Histochem Cytochem       Date:  2005-08-22       Impact factor: 2.479

3.  Elucidating the Burden of HIV in Tissues Using Multiplexed Immunofluorescence and In Situ Hybridization: Methods for the Single-Cell Phenotypic Characterization of Cells Harboring HIV In Situ.

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Journal:  J Histochem Cytochem       Date:  2018-02-20       Impact factor: 2.479

4.  The TALE class homeobox gene Smed-prep defines the anterior compartment for head regeneration.

Authors:  Daniel A Felix; A Aziz Aboobaker
Journal:  PLoS Genet       Date:  2010-04-22       Impact factor: 5.917

5.  Hind limb unloading of mice modulates gene expression at the protein and mRNA level in mesenchymal bone cells.

Authors:  Davide Visigalli; Antonella Strangio; Daniela Palmieri; Paola Manduca
Journal:  BMC Musculoskelet Disord       Date:  2010-07-05       Impact factor: 2.362

6.  Genoarchitecture of the extended amygdala in zebra finch, and expression of FoxP2 in cell corridors of different genetic profile.

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Journal:  Brain Struct Funct       Date:  2016-05-09       Impact factor: 3.270

7.  Genetic identification of the central nucleus and other components of the central extended amygdala in chicken during development.

Authors:  Alba Vicario; Antonio Abellán; Ester Desfilis; Loreta Medina
Journal:  Front Neuroanat       Date:  2014-09-10       Impact factor: 3.856

8.  A PCR-Based Method for RNA Probes and Applications in Neuroscience.

Authors:  Ruifang Hua; Shanshan Yu; Mugen Liu; Haohong Li
Journal:  Front Neurosci       Date:  2018-05-02       Impact factor: 4.677

Review 9.  Comprehensive autophagy evaluation in cardiac disease models.

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Journal:  Cardiovasc Res       Date:  2020-03-01       Impact factor: 10.787
  9 in total

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