Literature DB >> 1063412

Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues.

K A Gruber, S Stein, L Brink, A Radhakrishnan, S Udenfriend.   

Abstract

A fluorometric method for the quantitative assay of vasopressin and oxytocin in individual rat pituitaries has been developed. Acid extracts of pituitaries are freed of amino acids and polyamines by passage over a copper-Sephadex column, and the peptides fraction is then labeled by reaction with fluorescamine. The resulting peptide fluorophors are separated by chromatography on a reverse-phase bonded column. Specificity of the procedure was ascertained by several criteria, including bioassay and amino-acid analysis of the eluted peptide fluorophors. The procedure serves as a model system for the assay of tissue peptides in the picomole range.

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Year:  1976        PMID： 1063412      PMCID： PMC430258          DOI： 10.1073/pnas.73.4.1314

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  9 in total

1.  Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.

Authors:  P Böhlen; S Stein; J Stone; S Udenfriend
Journal:  Anal Biochem       Date:  1975-08       Impact factor: 3.365

2.  SEPARATION OF AMINO ACIDS, AS COPPER CHELATES, FROM AMINO ACID, PROTEIN AND PEPTIDE MIXTURES.

Authors:  S FAZAKERLEY; D R BEST
Journal:  Anal Biochem       Date:  1965-08       Impact factor: 3.365

3.  Removal of a Fluoram 1-positive impurity from hydrochloric acid.

Authors:  C Schwabe; J C Catlin
Journal:  Anal Biochem       Date:  1974-09       Impact factor: 3.365

4.  Amino acid analysis with fluorescamine of stained protein bands from polyacrylamide gels.

Authors:  S Stein; C H Chang; P Böhlen; K Imai; S Udenfriend
Journal:  Anal Biochem       Date:  1974-07       Impact factor: 3.365

5.  Amino acid analysis with fluorescamine at the picomole level.

Authors:  S Stein; P Böhlen; J Stone; W Dairman; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1973-03       Impact factor: 4.013

6.  A simplified protein assay with fluorescamine in samples containing interfering material.

Authors:  P Böhlen; S Stein; K Imai; S Udenfriend
Journal:  Anal Biochem       Date:  1974-04       Impact factor: 3.365

7.  Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.

Authors:  S Udenfriend; S Stein; P Böhlen; W Dairman; W Leimgruber; M Weigele
Journal:  Science       Date:  1972-11-24       Impact factor: 47.728

8.  Fluorescent labeling of proteins. A new methodology.

Authors:  M Weigele; S De Bernardo; W Leimgruber
Journal:  Biochem Biophys Res Commun       Date:  1973-10-01       Impact factor: 3.575

9.  Supraoptic neurosecretory neurons of the guinea pig in organ culture. Biosynthesis of vasopressin and neurophysin.

Authors:  H Sachs; R Goodman; J Osinchak; J McKelvy
Journal:  Proc Natl Acad Sci U S A       Date:  1971-11       Impact factor: 11.205
  9 in total
  4 in total

1.  A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues.

Authors:  H P Bennett; A M Hudson; L Kelly; C McMartin; G E Purdon
Journal:  Biochem J       Date:  1978-12-01       Impact factor: 3.857

2.  Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.

Authors:  M Rubinstein; S Stein; L D Gerber; S Udenfriend
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205

3.  Chemical determination of polypeptide hormones.

Authors:  K Tatemoto; V Mutt
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

4.  Axoplasmic transport of carnosine (β-alanyl-L-histidine) in the mouse olfactory pathway.

Authors:  F L Margolis; M Grillo
Journal:  Neurochem Res       Date:  1977-10       Impact factor: 3.996
  4 in total

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