Literature DB >> 233923

A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues.

H P Bennett, A M Hudson, L Kelly, C McMartin, G E Purdon.   

Abstract

Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography.

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Year:  1978        PMID： 233923      PMCID： PMC1186178          DOI： 10.1042/bj1751139

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  5 in total

1.  Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues.

Authors:  K A Gruber; S Stein; L Brink; A Radhakrishnan; S Udenfriend
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

2.  Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivo.

Authors:  H P Bennett; A M Hudson; C McMartin; G E Purdon
Journal:  Biochem J       Date:  1977-10-15       Impact factor: 3.857

3.  Tritiated peptides. Part 3. Synthesis of [4-3H-Phe7]-beta-corticotrophin-(1-24)-tetracosapeptide.

Authors:  D E Brundish; R Wade
Journal:  J Chem Soc Perkin 1       Date:  1976

4.  The synthesis of tritium-labelled human corticotropin of high specific radioactivity.

Authors:  D E Brundish; R Wade
Journal:  Biochem J       Date:  1977-07-01       Impact factor: 3.857

5.  Early fate of somatostatin in the circulation of the rat after intravenous injection.

Authors:  C McMartin; G E Purdon
Journal:  J Endocrinol       Date:  1978-04       Impact factor: 4.286
  5 in total
  6 in total

1.  Biological activities and chemical composition of purified tracheal cytotoxin of Bordetella pertussis.

Authors:  B T Cookson; H L Cho; L A Herwaldt; W E Goldman
Journal:  Infect Immun       Date:  1989-07       Impact factor: 3.441

2.  Identification of endogenous sympathetic neuron pituitary adenylate cyclase-activating polypeptide (PACAP): depolarization regulates production and secretion through induction of multiple propeptide transcripts.

Authors:  C A Brandenburg; V May; K M Braas
Journal:  J Neurosci       Date:  1997-06-01       Impact factor: 6.167

3.  The prenatal development profile of expression of opioid peptides and receptors in the mouse brain.

Authors:  R A Rius; J Barg; W T Bem; C J Coscia; Y P Loh
Journal:  Brain Res Dev Brain Res       Date:  1991-02-22

4.  Isolation and analysis of human parathyrin in parathyroid tissue and plasma. Use of reversed-phase liquid chromatography.

Authors:  H P Bennett; S Solomon; D Goltzman
Journal:  Biochem J       Date:  1981-08-01       Impact factor: 3.857

5.  PC13 embryonal carcinoma-derived growth factor.

Authors:  J K Heath; C M Isacke
Journal:  EMBO J       Date:  1984-12-01       Impact factor: 11.598

6.  Purification and characterization of a polypeptide from chick brain that promotes the accumulation of acetylcholine receptors in chick myotubes.

Authors:  T B Usdin; G D Fischbach
Journal:  J Cell Biol       Date:  1986-08       Impact factor: 10.539
  6 in total

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