Literature DB >> 1060119

Structure of transcriptionally active chromatin.

J M Gottesfeld, R F Murphy, J Bonner.   

Abstract

Rat-liver chromatin has bee fractionated into transcriptionally active and inactive regions [Gottesfeld et al. (1974) Proc. Nat. Acad. Sci. USA 71, 2193-2197] and the distribution of nuclease-resistant complexes in these fractions has been investigated. About half of the DNA of both fractions is resistant to attack by tne endonuclease DNase II. The nuclease-resistant structures of inactive chromatin are DNA-histone complexes (v-bodies) which sediment at 11-13 S. Template-active chromatin yields two peaks of nuclease-resistant nucleoprotein. These complexes sediment at 14 and 19 S, and contain DNA, RNA, histone, and nonhistone chromosomal proteins. Polyacrylamide gel electrophoresis reveals a complex pattern of chromatin proteins, suggesting that the complexes are heterogeneous in composition.

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Year:  1975        PMID： 1060119      PMCID： PMC388730          DOI： 10.1073/pnas.72.11.4404

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.

Authors:  P Oudet; M Gross-Bellard; P Chambon
Journal:  Cell       Date:  1975-04       Impact factor: 41.582

2.  Chromatin fragments resembling v bodies.

Authors:  M B Senior; A L Olins; D E Olins
Journal:  Science       Date:  1975-01-17       Impact factor: 47.728

3.  Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils.

Authors:  D K Oosterhof; J C Hozier; R L Rill
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

4.  Chemical probes of chromatin structure.

Authors:  R J Clark; G Felsenfeld
Journal:  Biochemistry       Date:  1974-08-13       Impact factor: 3.162

5.  Subunit structure of chromatin.

Authors:  M Noll
Journal:  Nature       Date:  1974-09-20       Impact factor: 49.962

6.  Biophysical studies on the mechanism of quinacrine staining of chromosomes.

Authors:  J M Gottesfeld; J Bonner; G K Radda; I O Walker
Journal:  Biochemistry       Date:  1974-07-02       Impact factor: 3.162

7.  Chromatin template activity and chromatin structure.

Authors:  R Axel; H Cedar; G Felsenfeld
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

8.  Spheroid chromatin units (v bodies).

Authors:  A L Olins; D E Olins
Journal:  Science       Date:  1974-01-25       Impact factor: 47.728

9.  Electron microscopy of chromatin subunit particles.

Authors:  E F van Bruggen; A C Arnberg; K E van Holde; C G Sahasrabuddhe; B R Shaw
Journal:  Biochem Biophys Res Commun       Date:  1974-10-23       Impact factor: 3.575

10.  Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis.

Authors:  L A Burgoyne; D R Hewish; J Mobbs
Journal:  Biochem J       Date:  1974-10       Impact factor: 3.857
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  35 in total

1.  Identification of nonhistone chromatin proteins in chromatin subunits.

Authors:  C C Liew; P K Chan
Journal:  Proc Natl Acad Sci U S A       Date:  1976-10       Impact factor: 11.205

2.  Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.

Authors:  A Garel; R Axel
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205

3.  Presence of messenger specifying sequences in the DNA of chromatin subunits.

Authors:  M Tien Kuo; C G Sahasrabuddhe; G F Saunders
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

4.  Distribution of estrogen receptors in subfractions of hen oviduct chromatin.

Authors:  K Hemminki
Journal:  Nucleic Acids Res       Date:  1976-06       Impact factor: 16.971

5.  Structure of transcriptionally-active chromatin subunits.

Authors:  J M Gottesfeld; P J Butler
Journal:  Nucleic Acids Res       Date:  1977-09       Impact factor: 16.971

6.  The absence of histone H1 from the chromatin fraction obtained by sonication of calf thymus nuclei under "quasiphysiological" ionic conditions.

Authors:  A I Lishanskaya; M I Mosevitsky
Journal:  Nucleic Acids Res       Date:  1976-08       Impact factor: 16.971

7.  Isolation and physicochemical characterisation of the DNA from normal human skin and psoriatic scales.

Authors:  G Teimer; G Ramb; G Leonhardi
Journal:  Arch Dermatol Res       Date:  1976-10-27       Impact factor: 3.017

8.  Conformational states of chromatin nu bodies induced by urea.

Authors:  D E Olins; P N Bryan; R E Harrington; W E Hill; A L Olins
Journal:  Nucleic Acids Res       Date:  1977-06       Impact factor: 16.971

9.  Morphology of transcription units in Drosophila melanogaster.

Authors:  C D Laird; W Y Chooi
Journal:  Chromosoma       Date:  1976-10-28       Impact factor: 4.316

10.  Tetrahymena ribosomal RNA gene chromatin is digested by micrococcal nuclease at sites which have the same regular spacing on the DNA as corresponding sites in the bulk nuclear chromatin.

Authors:  P W Piper; J Celis; K Kaltoft; J C Leer; O F Nielsen; O Westergaard
Journal:  Nucleic Acids Res       Date:  1976-02       Impact factor: 16.971
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