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Literature DB >> 1064026

Presence of messenger specifying sequences in the DNA of chromatin subunits.

M Tien Kuo, C G Sahasrabuddhe, G F Saunders.

Abstract

Messenger-specifying sequences in subunits of human lymphocyte chromatin were detected by hybridization of DNA complementary to cytoplasmic polyadenylylated RNA with DNA isolated from the subunits. Comparison of the kinetics and extents of hybridization of complementary DNA with chromatin subunit DNA and with nuclear DNA showed that most of the repetitive sequences and single copy sequences in mRNA are present in chromatin subunits. This result indicates that inclusion of a DNA sequence into the subunit does not prevent its transcription in vivo.

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Year: 1976 PMID： 1064026 PMCID： PMC430340 DOI： 10.1073/pnas.73.5.1572

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

Presence of messenger specifying sequences in the DNA of chromatin subunits.

1. Analysis of DNA of isolated chromatin subunits.

Review 2. Organization, transcription, and regulation in the animal genome.

3. Purification and detection of reverse transcriptase in viruses and cells.

4. Spheroid chromatin units (v bodies).

5. Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.

6. DNA-RNA hybridization in vast DNA excess.

7. Populations of repeated DNA sequences in the human genome.

8. Full length and discrete partial reverse transcripts of globin and chorion mRNAs.

9. The diversity of the messenger RNA population in growing Friend cells.

10. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

1. Transcription of nucleosomal DNA in SV40 minichromosomes by eukaryotic and prokaryotic RNA polymerases.

2. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.

3. Structure of transcriptionally-active chromatin subunits.

4. The sv40 transcription complex. II. Non-dissociation of protein from SV40 chromatin during transcription.

5. Features of the structure of replicating and non-replicating chromatin in chicken erythroblasts.

6. Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes.

7. Template active chromatin structures: degradation by deoxyribonuclease I.

8. Genes transcribed at diverse rates have a similar conformation in chromatin.

9. Presence of protein A24 in rat liver nucleosomes.

10. Binding of E. coli RNA polymerase to chromatin subunits.