Literature DB >> 183184

Distribution of estrogen receptors in subfractions of hen oviduct chromatin.

K Hemminki.   

Abstract

Hen oviduct chromatin was digested with DNase II and separated into two fractions. The MgCl2 insoluble chromatin fraction (43% of the total DNA) was enriched in nucleosome-like particles, which sedimented at 11 S and contained 185 base pairs of DNA. The MgCl2 soluble chromatin fraction (5% of the total DNA) was characterized by 5 S and 14 S peaks in sucrose gradients; Estrogen receptors in the chromatin fractions were labelled with [3H] estradiol using the steroid exchange assay. The concentration of receptors in the MgCl2 soluble chromatin was 4;5 times higher than that in the MgCl2 insoluble chromatinmin sucrose gradient analysis the 11 S particles displayed a negligible specific radioactivity suggesting that estrogen receptors mainly bind to extranucleosomal chromatin.

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Year:  1976        PMID： 183184      PMCID： PMC343008          DOI： 10.1093/nar/3.6.1499

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors:  T Maniatis; A Jeffrey; H van deSande
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

2.  Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.

Authors:  P Oudet; M Gross-Bellard; P Chambon
Journal:  Cell       Date:  1975-04       Impact factor: 41.582

3.  Cleavage of DNA in nuclei and chromatin with staphylococcal nuclease.

Authors:  R Axel
Journal:  Biochemistry       Date:  1975-07       Impact factor: 3.162

4.  A comparison of the digestion of nuclei and chromatin by staphylococcal nuclease.

Authors:  B Sollner-Webb; G Felsenfeld
Journal:  Biochemistry       Date:  1975-07       Impact factor: 3.162

5.  Structure of transcriptionally active chromatin.

Authors:  J M Gottesfeld; R F Murphy; J Bonner
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

6.  Electron microscopy of defined lengths of chromatin.

Authors:  J T Finch; M Noll; R D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

7.  Nucleas action on chromatin: evidence for discrete, repeated nucleoprotein units along chromatin fibrils.

Authors:  D K Oosterhof; J C Hozier; R L Rill
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

8.  Isolation of template active and inactive regions of chromatin.

Authors:  J Bonner; J Gottesfeld; W Garrard; R Billing; L Uphouse
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

9.  An octamer of histones in chromatin and free in solution.

Authors:  J O Thomas; R D Kornberg
Journal:  Proc Natl Acad Sci U S A       Date:  1975-07       Impact factor: 11.205

10.  Spheroid chromatin units (v bodies).

Authors:  A L Olins; D E Olins
Journal:  Science       Date:  1974-01-25       Impact factor: 47.728
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  2 in total

1.  Polylysine titration of rat liver chromatin fractions after DNase II digestion.

Authors:  M Vauhkonen; K Hemminki
Journal:  Nucleic Acids Res       Date:  1977-04       Impact factor: 16.971

2.  Oestradiol-receptor complexes in subnuclear fractions of rat uterine tissue.

Authors:  W de Boer; J de Vries; E Mulder; H J van der Molen
Journal:  Nucleic Acids Res       Date:  1978-01       Impact factor: 16.971
  2 in total

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