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Literature DB >> 4583025

Identification of 50S proteins at the peptidyl-tRNA binding site of Escherichia coli ribosomes.

H Oen, M Pellegrini, D Eilat, C R Cantor.

Abstract

Bromoacetyl-phenylalanyl-tRNA(phe) bound to 70S E. coli ribosomes reacts covalently with proteins of the 50S subunit. The major reactions are with proteins L2 and L27. In the presence of poly(U), 70S-bound bromoacetyl-phenylalanyl-tRNA(phe) can participate in peptidebond formation with phenylalanyl-tRNA(phe) or puromycin. Most of the products of these reactions are also found covalently attached to L2 and L27. Chloramphenicol and sparsomycin markedly inhibit the peptide-bond formation. These results strongly suggest that bromoacetylphenylalanyl-tRNA(phe) can function as a normal peptidyl-tRNA and that the 50S proteins, L2 and L27, are located in the peptidyl-tRNA binding site. The side reactions of bromoacetyl-phenylalanyl-tRNA(phe) are with one or more 50S proteins from the set L14-17, L6 and/or L11, and L26. These occur to a much less extent than the reactions with L2 and L27. Any functional significance of the side reactions is unknown.

Entities: Chemical Species

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Year: 1973 PMID： 4583025 PMCID： PMC427112 DOI： 10.1073/pnas.70.10.2799

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

16 in total

1. Ribosomal proteins. XXX. Specific protein binding sites on 23S RNA of Escherichia coli.

Authors: G Stöffler; L Daya; K H Rak; R A Garrett
Journal: Mol Gen Genet Date: 1972

2. Ribosomal proteins. XXIV. Trypsin digestion as a possible probe of the conformation of Escherichia coli ribosomes.

Authors: R R Crichton; H G Wittmann
Journal: Mol Gen Genet Date: 1972

3. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.

Authors: A Chrambach; R A Reisfeld; M Wyckoff; J Zaccari
Journal: Anal Biochem Date: 1967-07 Impact factor: 3.365

4. Reaction of N-ethyl maleimide with the ribosomes of Escherichia coli.

Authors: P B Moore
Journal: J Mol Biol Date: 1971-08-28 Impact factor: 5.469

5. Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.

Authors: M Dzionara; E Kaltschmidt; H G Wittmann
Journal: Proc Natl Acad Sci U S A Date: 1970-12 Impact factor: 11.205

6. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.

Authors: E Kaltschmidt; H G Wittmann
Journal: Anal Biochem Date: 1970-08 Impact factor: 3.365

7. Structure and function of E. coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and proteins.

Authors: P Traub; M Nomura
Journal: Proc Natl Acad Sci U S A Date: 1968-03 Impact factor: 11.205

8. Studies on rat liver phenylalanyl transfer ribonucleic acid synthetase. I. Purification, stabilization, and complex formation.

Authors: K W Lanks; J Sciscenti; I B Weinstein; C R Cantor
Journal: J Biol Chem Date: 1971-06-10 Impact factor: 5.157

8. Crosslinking of Escherichia coli 50S ribosomal subunits with chlorambucilyl oligoprolyl phenylalanyl-tRNA molecular rulers.

Authors: K K Parker; E Wickstrom
Journal: Nucleic Acids Res Date: 1983-01-25 Impact factor: 16.971

9. A new simpler photoaffinity analogue of peptidyl tRNA.

Authors: N Hsiung; C R Cantor
Journal: Nucleic Acids Res Date: 1974-12 Impact factor: 16.971

10. Photo-affinity labeling of tRNA binding sites in macromolecules. I. Linking of the phenacyl-p-azide of 4-thiouridine in (Escherichia coli) valyl-tRNA to 16S RNA at the ribosomal P site.

Authors: I Schwartz; J Ofengand
Journal: Proc Natl Acad Sci U S A Date: 1974-10 Impact factor: 11.205

10 in total

Identification of 50S proteins at the peptidyl-tRNA binding site of Escherichia coli ribosomes.

1. Ribosomal proteins. XXX. Specific protein binding sites on 23S RNA of Escherichia coli.

2. Ribosomal proteins. XXIV. Trypsin digestion as a possible probe of the conformation of Escherichia coli ribosomes.

3. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.

4. Reaction of N-ethyl maleimide with the ribosomes of Escherichia coli.

5. Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.

6. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.

7. Structure and function of E. coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and proteins.

8. Studies on rat liver phenylalanyl transfer ribonucleic acid synthetase. I. Purification, stabilization, and complex formation.

9. Ribosomal proteins of E. Coli: stoichiometry and implications for ribosome structure.

10. Ribosomal proteins. Isolation of proteins from 50S ribosomal subunits of Escherichia coli.

1. Identification of a ribosomal protein essential for peptidyl transferase activity.

2. Chloroplast ribosomal protein L-18 in Chlamydomonas reinhardtii is processed during ribosome assembly.

3. Affinity labeling of the ribosomal decoding site with an AUG-substrate analog.

4. Identification of a region in 23S rRNA located at the peptidyl transferase center.

5. Photoincorporation of puromycin and N-(ethyl-2-diazomalonyl)puromycin into Escherichia coli ribosomes.

6. Interactions between 30s ribosomal proteins and 50s subunits of Escherichia coli.

7. Mutational study of the rRNA in yeast mitochondria: functional importance of T1696 in the large rRNA gene.

8. Crosslinking of Escherichia coli 50S ribosomal subunits with chlorambucilyl oligoprolyl phenylalanyl-tRNA molecular rulers.

9. A new simpler photoaffinity analogue of peptidyl tRNA.

10. Photo-affinity labeling of tRNA binding sites in macromolecules. I. Linking of the phenacyl-p-azide of 4-thiouridine in (Escherichia coli) valyl-tRNA to 16S RNA at the ribosomal P site.